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Current Microbiology

, Volume 76, Issue 2, pp 258–262 | Cite as

Paracoccidioides spp. in Soil from the Pampa Biome in Southern Brazil

  • Josiara Furtado Mendes
  • Andrea Von Groll
  • Vanice Rodrigues Poester
  • Carolina Litchina Brasil
  • Tchana Martinez Brandolt
  • Gabriel Baracy Klafke
  • Carlos Eduardo Wayne Nogueira
  • Mário Carlos Araújo Meireles
  • Melissa Orzechowski XavierEmail author
Article

Abstract

High rates of paracoccidioidomycosis disease and infection have been recently reported in southern Brazil. However, the knowledgment about the ecoepidemiology of Paracoccidioides spp. in the region is limited, therefore, this study aimed to evaluate the presence of Paracoccidioides spp. DNA in soil samples from a rural area in Southern Rio Grande do Sul, Brazil. Thirty pools of soil samples from Bagé, RS (31º19′53"S 54º06′25"W) were submitted to physicochemical analysis, and to fungal DNA extraction by Norgen Biotek® Kit (Thorold, Canada), followed by Nested PCR technique with ITS4 and ITS5 as external primers, and PBITS-E and PBITS-T as internal primers. DNA amplification products of about 424 bp compatible with Paracoccidioides spp. were detected in eight (26.7%) of the 30 pools of samples, being three were sequenced and identified as P. brasiliensis. Positive soils were characterized by high levels of humidity, organic matter, basic saturation, and pH. This study shows for the first time the presence of Paracoccidioides spp. DNA at soils from the Brazilian Pampa Biome, proving that people living in those areas are exposed to the main agent of paracoccidioidomycosis.

Notes

Acknowledgements

We thank Coordenação de Aperfeiçoamento Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico (CNPq), and Prof. Anderson Messias Rodrigues, Prof. Zoilo Pires de Camargo, and Msc. Paula Portella Della Terra of Laboratory of Cellular and Molecular Immunology of UNIFESP.

Compliance with Ethical Standards

Conflict of interest

All authors declare that they have no conflicts of interest pertaining to this work.

Ethical Approval

All procedures performed in studies were in accordance with the ethical standards of the institution.

Informed consent

Informed consent was obtained from all individual participants reported in the study.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Josiara Furtado Mendes
    • 1
  • Andrea Von Groll
    • 2
  • Vanice Rodrigues Poester
    • 2
    • 3
  • Carolina Litchina Brasil
    • 4
  • Tchana Martinez Brandolt
    • 2
    • 3
  • Gabriel Baracy Klafke
    • 3
  • Carlos Eduardo Wayne Nogueira
    • 1
  • Mário Carlos Araújo Meireles
    • 1
  • Melissa Orzechowski Xavier
    • 2
    • 3
    • 4
    Email author
  1. 1.Post-Graduation Program in VeterinaryFederal University of Pelotas (UFPel)PelotasBrazil
  2. 2.Post-Graduation Program in Health ScienceFederal University of Rio Grande (FURG)Rio GrandeBrazil
  3. 3.Mycology Lab, Faculty of MedicineFederal University of Rio Grande (FAMED-FURG)Rio GrandeBrazil
  4. 4.Post-Graduation Program in Parasitology UFPelPelotasBrazil

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