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Tepidicella baoligensis sp. nov., A Novel Member of Betaproteobacterium Isolated from an Oil Reservoir

  • Jing You
  • Yongbin Li
  • Shan Hong
  • Jing Wang
  • Jiliang Yu
  • Bozhong MuEmail author
  • Xiaochen MaEmail author
  • Yanfen Xue
Article
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Abstract

A Gram-negative, non-pigmented, aerobic bacterium, designated strain B18-50T was isolated from oil-well production water in Baolige oilfield, China. The strain was able to grow at pH 6.5–10.5 (optimum at pH 7.5–8.5), in 0–3% (w/v) NaCl (optimum at 0–0.5%, w/v) and at 20–60 °C (optimum at 45 °C). Cells of the isolate were motile with a single polar flagellum and non-spore-forming rods. Organic acids and amino acids were used as carbon and energy sources, but sugars and polyols were not assimilated. The major cellular fatty acids were C16:0, C16:1ω6c/ω7c, and C18:1ω7c. Ubiquinone 8 was the predominant respiratory quinone. The major polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol. The genomic DNA G+C content of the isolate was 62.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain B18-50T was most closely related to Tepidicella xavieri DSM 19605T (97.5% similarity). Comparative analysis of genotypic and phenotypic features indicate that strain B18-50T represents a novel species of the genus Tepidicella, for which the name Tepidicella baoligensis sp. nov. is proposed. The type strain is B18-50T (= CGMCC 1.13575T = KCTC 62779T).

Notes

Acknowledgements

This work was supported by the China National Petroleum Corporation (Grant Number 2017E-1507) and the Huabei Oilfield Company (Grant Number 2017-HB-B19).

Compliance with Ethical Standards

Conflict of interest

The authors have declared that no competing interests exist.

Ethical approval

The authors have declared that no ethical issues exist.

Supplementary material

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Supplementary material 1 (DOCX 1059 KB)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.School of Chemistry and Molecular EngineeringEast China University of Science and TechnologyShanghaiChina
  2. 2.Institute of MicrobiologyCASBeijingChina
  3. 3.Engineering Technology Research Institute of Huabei Oilfield CompanyRenqiuChina

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