Biofilm Formation in Different Salmonella Serotypes Isolated from Poultry
- 100 Downloads
Little is known about Salmonella biofilm assembly, making the prevention of the disease a challenge in the poultry production chain. The objective of the present study was then to evaluate biofilm formation from different serotypes of Salmonella spp. in both polystyrene plates and eggshells. Salmonella Gallinarum and S. Minnesota were both classified as producers of biofilms of moderate intensity. Interestingly, S. Gallinarum produces biofilm even though being a serotype without flagellum and not having the lux gene in its genome, suggesting that there might be other important structures and genes associated with biofilm formation. Regarding Enteritidis, Typhimurium, Typhimurium variant, and Heidelberg serotypes, despite having high counts, BFI (Biofilm Formation Index) showed low biofilm production, probably due to the scarcity of extracellular matrix produced by such strains. A turkey eggshell model was then used for S. Enteritidis and S. Heidelberg biofilm formation. The results from the microbial count and scanning electron microscopy showed that Salmonella serotypes were also able to generate biofilm in eggshells, suggesting the presence of biofilms in poultry producing farms, a main concern for the poultry production industry.
The authors gratefully acknowledge the support of the CNPq for their financial support.
- 3.Centers for Disease Control –CDC (2014) Multistate Outbreak of Multidrug-Resistant Salmonella Heidelberg Infections Linked to Foster Farms Brand ChickenGoogle Scholar
- 4.Center for Disease Control-CDC (2016) Making food safer to eat: reducing contamination from the farm to the table. http://www.cdc.gov/vitalsigns/foodsafety/. Accessed 07 May 2018
- 8.Li J (2016) The effects of meat juice on biofilm formation of Campylobacter and Salmonella. Thesis, The University of British ColumbiaGoogle Scholar
- 12.Sanjay MK, Shrideshikan SM, Usha MS, Philipraj A, Gaddad SM, Shivannavar CT (2010) Detection, amplification & sequence homology of sodC in clinical isolates of Salmonella sp. Indian J Med Res 131:565–570Google Scholar
- 15.Sukupolvi S, Lorenz RG, Gordon JI, Bian Z, Pfeifer JD, Normark SJ, Rhen M (1997) Expression of thin aggregative fimbriae promotes interaction of Salmonella Typhimurium SR-11 with mouse small intestinal epithelial cells. Infect Immun 65:5320–5325. https://doi.org/PMID:9393832Google Scholar
- 17.Sun J, Hobert ME, Rao AS, Neish AS, Madara JL (2004) Bacterial activation of -catenin signaling in human epithelia. Am J Physiol Gastrointest Liver Physiol 287:220 – 227. https://doi.org/PMCID:PMC356064
- 19.Turki Y, Ouzari H, Khessairi A, Hassen A (2014) Molecular typing, antibiotic resistance, virulence gene and biofilm formation of different Salmonella enterica serotypes. J Gen Appl Microbiol 60:123–130. https://doi.org/PMID 25273985
- 21.World Health Organization – WHO (2011) Critically Important Antimicrobials for Human Medicine. 3rd Revision. SwitzerlandGoogle Scholar