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Current Microbiology

, Volume 76, Issue 2, pp 133–143 | Cite as

Wolbachia Population in Vectors and Non-vectors: A Sustainable Approach Towards Dengue Control

  • Ipsita Mohanty
  • Animesha Rath
  • Swayam Prava Swain
  • Nitika Pradhan
  • Rupenangshu Kumar HazraEmail author
Article

Abstract

Wolbachia is gram negative obligate endosymbiont known for reproductive manipulation in the host. It is important to study the presence of natural Wolbachia in mosquitoes which can later help in understanding the effect of transfected strain on indigenous strain. With this view, the present study is undertaken to focus on the prevalence, diversity, infection frequencies, phylogeny and density of indigenous Wolbachia strains in wild mosquito species of Odisha. Our study confirms Wolbachia presence in Ae. albopictus, Cx. quinquefasciatus, Cx. vishnui, Cx. gelidus, Ar. subalbatus, Mn. uniformis, and Mn. indiana. Wolbachia in the above mosquitoes were separated into two supergroups (A and B). Ae. albopictus, the major vector of dengue and chikungungunya had both super-infection and mono-infection. The ovaries of Ae. albopictus were highest in density of Wolbachia as compared to midguts or salivary glands. wAlBA and wAlbB density were variable in mosquitoes of F1 generation for both the sex and at different age. We also found that Wolbachia super-infection in females tends to increase whereas wAlbA density reduced completely as compared to wAlbB in males when they grew old. Giemsa stained squashed ovaries revealed pink pleomorphic Wolbachia cells with different shapes and forms. This study is unique in its kind covering the major aspects of the endosymbiont Wolbachia and focusing on its potential as a biocontrol agent in arboviral outbreaks. Knowledge on potential of the indigenous strain and interactions between Wolbachia and viruses can be utilized further to reduce the global burden of vector borne diseases.

Notes

Acknowledgements

We are grateful to the Director, RMRC for providing a platform for this study. We thank Director, NVBDCP, Bhubaneswar and staff for sharing data for this study. We thank insectarium staff Ms Santoshini Dash and Ms Jyotiprabha Garanayak of RMRC, Bhubaneswar for technical help. We are extremely delightful to thank Lady Tata Memorial Trust, Mumbai, for providing scholarship for PhD to Miss Ipsita Mohanty.

Funding

This work is funded by Lady Tata Memorial Trust, Mumbai, India and Indian Council of Medical Research, New Delhi, India.

Compliance with Ethical Standards

Conflict of interest

The authors declare no conflicts of interest.

Ethical Approval

All procedures performed in the study involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Regional Medical Research Centre (ICMR)BhubaneswarIndia
  2. 2.KIIT School of BiotechnologyKalinga Institute of Industrial TechnologyBhubaneswarIndia
  3. 3.Odisha University of Agriculture and TechnologyBhubaneswarIndia
  4. 4.Division of Medical Entomology, Regional Medical Research CentreIndian Council of Medical ResearchBhubaneswarIndia

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