Current Microbiology

, Volume 76, Issue 1, pp 95–99 | Cite as

Transcriptome-Based Identification of a Strong Promoter for Hyper-production of Natamycin in Streptomyces

  • Kai Wang
  • Xiao-Fang Liu
  • Qing-Ting Bu
  • Yang Zheng
  • Xin-Ai Chen
  • Yong-Quan LiEmail author
  • Xu-Ming MaoEmail author


Streptomyces are famed producers of secondary metabolites with diverse bioactivities and structures. However, biosynthesis of natural products will consume vast precursors from primary metabolism, and some secondary metabolites are toxic to the hosts. To overcome this circumstance and over-produce secondary metabolites, one of the strategies is to over-express biosynthetic genes under strong promoters specifically expressed during secondary metabolism. For this purpose, here based on Microarray and eGFP reporter assays, we obtained a promoter thlM4p, whose activity was undetectable in the first 2 days of fermentation, but sevenfold higher than the strong promoter ermE*p in the following days. Moreover, when the positive regulator gene scnRII was driven from thlM4p, natamycin yield increased 30% compared to ermE*p. Therefore, we provide a new way to identify promoters, which is silenced during primary metabolism while strongly expressed under secondary metabolism of Streptomyces.



This work was financially supported by National Key Research and Development Program (2016YFD0400805), Natural Science Foundation of China (31571284, 31470212, 31520103901).

Supplementary material

284_2018_1589_MOESM1_ESM.pdf (1 mb)
Supplementary material 1 (PDF 1027 KB)


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Institute of Pharmaceutical BiotechnologyZhejiang UniversityHangzhouChina
  2. 2.Zhejiang Provincial Key Laboratory for Microbial Biochemistry and Metabolic EngineeringHangzhouChina

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