Halorubrum glutamatedens sp. nov., a Halophilic Archaeon Isolated from a Rock Salt
An extremely halophilic archaeon, strain ZY8T, was isolated from a rock salt of Yunnan salt mine. It was able to grow at 12–30% (w/v) NaCl (optimum, 15–20%), pH 7.0–9.0 (optimum, pH 8.5), and 20–45 °C (optimum, 42 °C). Sequence similarity search of its 16S rRNA gene showed that strain ZY8T belonged to the genus Halorubrum, and it is closely related to species of H. aethiopicum SAH-A6T (98.6%), H. aquaticum EN-2T (98.6%), and H. halodurans Cb34T (98.5%), respectively. Strain ZY8T contained phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and phosphatidylglycerol sulfate as its major phospholipids, and a sulfated diglycosyl diether as its major glycolipid. The DNA G+C content was 66.7 mol%. DNA–DNA relatedness between strains ZY8T and closely related species were far below 70%. Based on the phenotypic and phylogenetic analyses, it is proposed that strain ZY8T represents a novel species of the genus Halorubrum, for which the name Halorubrum glutamatedens sp. nov. is proposed. The type strain is ZY8T (=CGMCC 1.16026T=NBRC 112866T).
This work was supported by grants from the National Natural Science Foundation of China (31460003), the Anhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources (591601), the Anhui Provincial Natural Science Research Project (KJ2017A318) and the Education Department of Anhui province (gxyqZD2017011).
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