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Halorubrum glutamatedens sp. nov., a Halophilic Archaeon Isolated from a Rock Salt

  • Yao Xu
  • Jinting Lv
  • Chizhen Xie
  • Siqi Sun
  • Lixia Ke
  • Shaoxing Chen
Article

Abstract

An extremely halophilic archaeon, strain ZY8T, was isolated from a rock salt of Yunnan salt mine. It was able to grow at 12–30% (w/v) NaCl (optimum, 15–20%), pH 7.0–9.0 (optimum, pH 8.5), and 20–45 °C (optimum, 42 °C). Sequence similarity search of its 16S rRNA gene showed that strain ZY8T belonged to the genus Halorubrum, and it is closely related to species of H. aethiopicum SAH-A6T (98.6%), H. aquaticum EN-2T (98.6%), and H. halodurans Cb34T (98.5%), respectively. Strain ZY8T contained phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and phosphatidylglycerol sulfate as its major phospholipids, and a sulfated diglycosyl diether as its major glycolipid. The DNA G+C content was 66.7 mol%. DNA–DNA relatedness between strains ZY8T and closely related species were far below 70%. Based on the phenotypic and phylogenetic analyses, it is proposed that strain ZY8T represents a novel species of the genus Halorubrum, for which the name Halorubrum glutamatedens sp. nov. is proposed. The type strain is ZY8T (=CGMCC 1.16026T=NBRC 112866T).

Notes

Acknowledgements

This work was supported by grants from the National Natural Science Foundation of China (31460003), the Anhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources (591601), the Anhui Provincial Natural Science Research Project (KJ2017A318) and the Education Department of Anhui province (gxyqZD2017011).

Supplementary material

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References

  1. 1.
    Amoozegar MA, Siroosi M, Atashgahi S, Smidt H, Ventosa A (2017) Systematics of haloarchaea and biotechnological potential of their hydrolytic enzymes. Microbiology 163:623–645CrossRefGoogle Scholar
  2. 2.
    Chen S, He J, Zhang J, Xu Y, Huang J, Ke LX (2017) Halorubrum salsamenti sp. nov., a novel nalophilic archaeon isolated from a brine of salt mine. Curr Microbiol 74:1358–1364CrossRefGoogle Scholar
  3. 3.
    Chen S, Sun SQ, Xu Y, Lv JT, Chen LN, Liu L (2017) Halorubrum depositum sp. nov., a novel halophilic archaeon isolated from a salt deposit. Curr Microbiol 75:677–683CrossRefGoogle Scholar
  4. 4.
    De la Haba RR, Corral P, Sánchez-Porro C, Infante-Domínguez C, Makkay AM, Amoozegar MA, Ventosa A, Papke RT (2018) Genotypic and lipid analyses of strains from the archaeal genus Halorubrum reveal insights into their taxonomy, divergence, and population structure. Front Microbiol 9:512CrossRefGoogle Scholar
  5. 5.
    De Ley J, Cattoir H, Reynaerts A (1970) The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142CrossRefGoogle Scholar
  6. 6.
    Fan H, Xue Y, Ma Y, Ventosa A, Grant WD (2004) Halorubrum tibetense sp. nov., a novel haloalkaliphilic archaeon from Lake Zabuye in Tibet, China. Int J Syst Evol Microbiol 54:1213–1216CrossRefGoogle Scholar
  7. 7.
    Gibtan A, Song HS, Kim JY, Kim YB, Park N, Park K, Lee SJ, Kwon J, Roh SW, Lee HS (2018) Halorubrum aethiopicum sp. nov., an extremely halophilic archaeon isolated from commercial rock salt. Int J Syst Evol Microbiol 68:416–422CrossRefGoogle Scholar
  8. 8.
    Kamekura M (1993) The biology of halophilic bacteria. Lipids of extreme halophiles. CRC Press, Boca Raton, pp 135–161Google Scholar
  9. 9.
    McGenity TJ, Grant WD (1995) Transfer of Halobacterium saccharovorum, Halobacterium sodomense, Halobacterium trapanicum NRC 34021 and Halobacterium lacusprofundi to the Genus Halorubrum gen. nov., as Halorubrum saccharovorum comb. nov., Halorubrum sodomense comb. nov., Halorubrum trapanicum comb. nov., and Halorubrum lacusprofundi comb. nov. Syst Appl Microbiol 18:237–243CrossRefGoogle Scholar
  10. 10.
    McGenity TJ, Grant WD (2001) Genus VII. In: Halorubrum. Bergey’s manual of systematic bacteriology. Springer, New York, pp 320–324Google Scholar
  11. 11.
    McGenity TJ, Oren A (2012) Life in saline environments. In: BellE (ed) Life at extremes: environments, organisms, and strategiesfor survival. CABI International, Wallingford, pp 402–437CrossRefGoogle Scholar
  12. 12.
    Oren A, Ventosa A, Grant WD (1997) Proposed minimal standards for description of new taxa in the order Halobacteriales. Int J Syst Bacteriol 47:233–238CrossRefGoogle Scholar
  13. 13.
    Parte AC (2018) LPSN—list of prokaryotic names with standing in nomenclature (bacterio.net), 20 years on. Int J Syst Evol Microbiol 68:1825–1829CrossRefGoogle Scholar
  14. 14.
    Qiu XX, Zhao ML, Han D, Zhang WJ, Cui HL (2013) Halorubrum rubrum sp. nov., an extremely halophilic archaeon from a Chinese salt lake. Antonie Van Leeuwenhoek 104:885–891CrossRefGoogle Scholar
  15. 15.
    Stackebrandt E, Goebel BM (1994) Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849CrossRefGoogle Scholar
  16. 16.
    Tamaoka J, Komagata K (1984) Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128CrossRefGoogle Scholar
  17. 17.
    Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739CrossRefGoogle Scholar
  18. 18.
    Vreeland RH, Rosenzweig WD, Powers DW (2000) Isolation of a 250 million-year-old halotolerant bacterium from a primary salt crystal. Nature 407:897–900CrossRefGoogle Scholar
  19. 19.
    Xiao W, Wang ZG, Wang YX, Schneegurt MA, Li ZY, Lai YH, Zhang SY, Wen ML, Cui XL (2013) Comparative molecular analysis of the prokaryotic diversity of two salt mine soils in southwest China. J Basic Microbiol 53:942–952CrossRefGoogle Scholar
  20. 20.
    Yoon SH, Ha SM, Kwon S, Lim J, Kim Y, Seo H, Chun J (2017) Introducing EzBioCloud: a taxonomically united database of 16S rRNA and whole genome assemblies. Int J Syst Evol Microbiol 67:1613–1617CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Yao Xu
    • 1
  • Jinting Lv
    • 1
  • Chizhen Xie
    • 1
  • Siqi Sun
    • 1
  • Lixia Ke
    • 1
  • Shaoxing Chen
    • 1
    • 2
  1. 1.College of Life SciencesAnhui Normal UniversityWuhuPeople’s Republic of China
  2. 2.College of Life SciencesHonghe UniversityMengziPeople’s Republic of China

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