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cAMP Receptor Protein of Salmonella enterica Serovar Typhimurium Modulate Glycolysis in Macrophages to Induce Cell Apoptosis

  • Ke Ding
  • Chunjie Zhang
  • Jing Li
  • Songbiao Chen
  • Chengshui Liao
  • Xiangchao Cheng
  • Chuang Yu
  • Zuhua Yu
  • Yanyan Jia
Article
  • 117 Downloads

Abstract

We studied the role of glycolysis in the mechanism of cAMP receptor protein-induced macrophage cell death of Salmonella enterica serovar Typhimurium (S. Typhimurium). Cell apoptosis, caspase-3, -8, -9 enzyme activity, and pyruvic acid, lactic acid, ATP, and hexokinase (HK) contents were determined after infection of macrophages with S. Typhimurium SL1344 wild-type and a cAMP receptor protein mutant strain. While cell apoptosis, caspase-3, -8, -9 enzyme activity, lactic acid, hexokinase, and ATP levels significantly changed by infection with crp mutants compared to the wild-type strain (P < 0.05). Our data suggest that the cAMP receptor protein of S. Typhimurium can modulate macrophage death by effecting glycolysis levels. This finding may help to elucidate the mechanisms of S. Typhimurium pathogenesis.

Notes

Acknowledgements

This study was supported by grants from the National Natural Science Foundation of China (Grant 31572489), the HeNan Natural Science Foundation (182300410078), and the National Key R&D Program (2016YFD0500708).

Compliance with Ethical Standards

Conflict of interest

The authors or their institution do not have any relationships that may influence or bias the results and data presented in this manuscript.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Ke Ding
    • 1
    • 2
  • Chunjie Zhang
    • 1
    • 2
  • Jing Li
    • 1
    • 2
  • Songbiao Chen
    • 1
    • 2
  • Chengshui Liao
    • 1
    • 2
  • Xiangchao Cheng
    • 1
    • 2
  • Chuang Yu
    • 1
    • 2
  • Zuhua Yu
    • 1
    • 2
  • Yanyan Jia
    • 1
    • 2
  1. 1.The Key Lab of Animal Disease and Public HealthyHenan University of Science and TechnologyLuoyangChina
  2. 2.Luoyang Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and ControlLuoyangChina

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