Current Microbiology

, Volume 75, Issue 12, pp 1634–1641 | Cite as

Transcriptional Regulation Between the Two Global Regulators RovA and CRP in Yersinia pestis biovar Microtus

  • Lei Liu
  • Haihong Fang
  • Yinjuan Ding
  • Yaqiong Zheng
  • Liping Cai
  • Shangen ZhengEmail author
  • Yiquan ZhangEmail author


Yersinia pestis is a dangerous bacterial pathogen that can cause plague. Both RovA and cyclic AMP receptor protein (cAMP-CRP) are required for regulating biofilm- and virulence-related genes in Y. pestis. In this study, the transcriptional regulation between RovA and cAMP-CRP were analyzed by using primer extension, quantitative RT-PCR, LacZ fusion, and electrophoretic mobility shift assay. The results indicated that RovA repressed crp transcription in an indirect manner, while that RovA had no regulatory action on cyaA at the transcriptional level. In addition, cAMP-CRP did not regulate the transcription of rovA. Taken together with our previous results, complex regulatory interactions of RovA, cAMP-CRP, and PhoP/PhoQ in Y. pestis were revealed, which would promote us gain deeper understanding about coordinative modulation of biofilm- and virulence-related regulator genes.



This work was supported by the National Natural Science Foundation of China (81801984), and by the Natural Science Foundation of Hubei Province (2018CFB184), and by the Hubei Province health and family planning scientific research project (WJ2018H0070).

Supplementary material

284_2018_1571_MOESM1_ESM.docx (426 kb)
Supplementary material 1 (DOCX 425 KB)


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of TransfusionWuhan General Hospital of PLAWuhanChina
  2. 2.State Key Laboratory of Pathogen and BiosecurityBeijing Institute of Microbiology and EpidemiologyBeijingChina
  3. 3.School of MedicineJiangsu UniversityZhenjiangChina

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