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Effects of Glucose Concentration on Ethanol Fermentation of White-Rot Fungus Phanerochaete sordida YK-624 Under Aerobic Conditions

  • Toshio Mori
  • Ojiro Kondo
  • Hirokazu Kawagishi
  • Hirofumi HiraiEmail author
Article
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Abstract

White-rot fungi are microorganisms capable of ethanol fermentation; however, the specific conditions activating ethanol fermentation are unclear in contrast to fermentation by yeasts. In this study, we investigated the conditions favoring ethanol fermentation by the white-rot fungus Phanerochaete sordida YK-624, which is able to produce ethanol from woody material. In aerobic stationary cultivation with various concentrations of glucose (0.8–33 g/l), the fungus produced ethanol in media containing an initial glucose concentration of 2.8 g/l or higher. The amount of glucose consumption, mycelial weight, and ethanol production on the second day of culture increased in a concentration-dependent manner at low glucose concentrations; however, these were saturated at high concentrations. Biomass yields (growth/glucose consumption) were decreased until the initial glucose concentration increased to 6.0 g/l, after which the biomass yields showed constant values at higher concentrations (12–33 g/l). On the other hand, ethanol yields increased with decreasing biomass yields. In short shaking cultivation using mycelial suspension, trace amounts of instantaneous aerobic ethanol production were observed with 1.1 and 2.1 g/l glucose, but the relative gene expression levels of key enzymes at the pyruvate branch point showed no significant differences between ethanol production and non-production conditions. From these experimental results, it appears that the white-rot fungus P. sordida YK-624 produces ethanol due to overflow in sugar metabolism under aerobic conditions, although P. sordida YK-624 prioritizes glucose utilization for respiratory growth.

Notes

Acknowledgements

This work was supported by a Grant-in-Aid for Scientific Research (C) (Grant No. 17K08167) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

284_2018_1622_MOESM1_ESM.docx (324 kb)
Supplementary material 1 (DOCX 323 KB)

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Toshio Mori
    • 1
  • Ojiro Kondo
    • 1
  • Hirokazu Kawagishi
    • 1
    • 2
    • 3
  • Hirofumi Hirai
    • 1
    • 2
    Email author
  1. 1.Faculty of AgricultureShizuoka UniversityShizuokaJapan
  2. 2.Research Institute of Green Science and TechnologyShizuoka UniversityShizuokaJapan
  3. 3.Graduate School of Science and TechnologyShizuoka UniversityShizuokaJapan

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