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Current Microbiology

, Volume 72, Issue 2, pp 228–233 | Cite as

Loktanella aquimaris sp. nov., Isolated from Seawater

  • Kunho Kim
  • Sathiyaraj Srinivasan
  • Sang-Seob LeeEmail author
Article

Abstract

Two novel bacterial strains, designated C5T and C9 were isolated from a fish cage at Tongyeong, South Korea and were characterized to determine their taxonomic position. The strains were Gram-negative, non-motile, strictly aerobic and short rod shaped. Growth occurred between 20 and 32 °C (optimum 30 °C) and at pH 6.0–9.0 (optimum pH 8.0) and at 0–10 % NaCl (optimum at 2 % NaCl). Based on the 16S rRNA gene sequence analysis, they were identified as a member of the genus Loktanella that belongs to the phylum Proteobacteria. Strains C5T and C9 were analyzed by a polyphasic approach, revealing variations in their phenotypic characters but reciprocal DNA–DNA hybridization values confirmed that they belong to the same species. Phylogenetic analysis of the 16S rRNA gene sequences of closely related species indicated their similarities were below 97 %. The predominant isoprenoid quinone is ubiquinone-10 (Q-10). The major cellular fatty acids were C16:0, and C18:1 ω7c. Major polar lipid contained phosphatidylglycerol (PG), phosphatidycholine (PC), diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), unidentified amino lipid (AL) and unidentified lipids (L1−3). Based on phylogenetic and phenotypic data, the isolated strains represent a novel species of the genus Loktanella, for which the name Loktanella aquimaris sp. nov. is proposed. The type strains were C5T (=KEMB 3-892T = JCM 30382T), and a second strain is C9 (=KEMB 3-893 = JCM 30383).

Keywords

Bacteria Loktanella Polyphasic Taxonomy 

Notes

Acknowledgments

This research is supported by National Research foundation of Korea (NRF-2013M3A2A1067498) and (NRF-2010-0007473).

Supplementary material

284_2015_945_MOESM1_ESM.docx (119 kb)
Supplementary material 1 (DOCX 119 kb)

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Kunho Kim
    • 1
  • Sathiyaraj Srinivasan
    • 2
  • Sang-Seob Lee
    • 2
    Email author
  1. 1.Department of Biological Engineering, Graduate schoolKyonggi UniversitySuwonRepublic of Korea
  2. 2.Department of Life Science, College of Natural ScienceKyonggi UniversitySuwonRepublic of Korea

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