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Application of a digital PCR method for WT1 to myeloid neoplasms in CR and deep ELN WT1 molecular response (< 10 copies)

Abstract

Bone marrow WT1 mRNA levels assessed by the ELN method are useful to establish prognostic correlations in myeloid malignancies treated with chemotherapy or hematopoietic stem cell transplantation (HCT). Those patients with WT1 levels below ten copies have a good outcome. However, some of these patients relapse. To further characterize this group of cases, we applied a new and sensitive digital (ddPCR) WT1 method. A consecutive series of 49 patients with treated myeloid malignancies and with an ELN WT1 quantitation of < 10 copies were included in the study. All cases (47 AML and 2 MDS) have received intensive chemotherapy or HCT. One to four micrograms of total RNA were retrotranscribed to obtain ≥ 10,000 ABL1 copies using the ELN protocol. Only those cases with a good quality cDNA were used in the ddPCR WT1 test. The ddPCR Gene Expression WT1 Assay of Bio-Rad© was used to perform the PCR amplification, and the microdroplets were quantified in the Bio-Rad’s QX200 droplet reader. Eighteen patients showed a negative WT1 ddPCR assay (0 copies/μl), whereas 31 cases were positive (results ranged from 1 to 15.2 copies/μl). Survival analysis showed statistically significant differences in terms of OS between both groups, 83 ± 8% vs. 46 ± 9% (p = 0.024). A statistically significant correlation was also found between ddPCRWT1 results and CD123+ cell number detected by flow cytometry (p = 0.024). Larger series of patients tested with the current ddPCRWT1 method will solve whether it could be used to stratify patients with myeloid malignancies achieving deep WT1 molecular response (< 10 copies).

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Funding

The following grants funded this work: Fundación Mutua Madrileña 08/FMMA; PI13/2729, PI16/094 from the Instituto de Salud Carlos III, Ministerio de Economía y Competitividad, Spain; 2014-SGR-383, 2017-SGR-1395, PERIS SLT 002/16/0043 from Plà de Recerca de Catalunya; and a grant from Fundacion Josep Carreras and “Obra Social La Caixa” Barcelona Spain.

Author information

Correspondence to J. Nomdedéu.

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Conflict of interest

Josep Nomdedeu (JN) received honoraria from Novartis. The rest of the authors (EB, MP, MC, LS, MAR, AM, AB, IB, JE, MA, CT, MT, AG, FV, XO, CP, JB, SB, and JS) had nothing to disclose.

Ethical approval

All procedures were following the ethical standards of the Hospital de Sant Pau research committee, followed the SEHH recommendations, and were in accordance with the 1964 Helsinki declaration and its later amendments.

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Informed consent was obtained from all individual participants included in the study.

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Bussaglia, E., Pratcorona, M., Carricondo, M. et al. Application of a digital PCR method for WT1 to myeloid neoplasms in CR and deep ELN WT1 molecular response (< 10 copies). Ann Hematol (2020). https://doi.org/10.1007/s00277-020-03910-0

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Keywords

  • Leukemia
  • WT1
  • Molecular diagnostics
  • Minimal residual disease
  • Digital PCR