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World Journal of Surgery

, Volume 34, Issue 11, pp 2677–2681 | Cite as

In vitro Effectiveness of Garlic (Allium sativum) Extract on Scolices of Hydatid Cyst

  • Mohammad MoazeniEmail author
  • Ali Nazer
Article

Abstract

Background

Surgery is still the main treatment for hydatid disease. Recurrence of the infection is one of the end points of surgery in treating the hydatid cyst which results from the dissemination of protoscolices-rich fluid. Installation of a scolicidal agent into the cyst is the most commonly employed measure to prevent recurrence. Many scolicidal agents have been used for inactivation of the cyst’s content, but most of them are not safe due to their undesired side effects. In the present study, the scolicidal effect of methanolic extract of Allium sativum is investigated.

Methods

Protoscolices were aseptically collected from sheep livers containing hydatid cysts. Two concentrations (25 and 50 mg ml−1) of garlic extract were used for 10, 20, 30, 40, 50, and 60 min. Viability of protoscolices was confirmed by 0.1% eosin staining.

Results

Allium sativum extract at the concentration of 25 mg ml−1 killed 87.9, 95.6, 96.8, 98.7, 99.6, and 100% of protoscolices following 10, 20, 30, 40, 50, and 60 min of application, respectively. Moreover, the scolicidal activity of Allium sativum extract at the concentration of 50 mg ml−1 was 100% after 10 min of application.

Conclusions

Methanolic extract of Allium sativum had a high scolicidal activity in vitro and thus might be used as a scolicidal agent in the surgical treatment of the hydatid cyst. However, further investigation on the in vivo efficacy of Allium sativum extract and its possible side effects is proposed.

Keywords

Methanolic Extract Hydatid Cyst Hypertonic Saline Echinococcosis Cystic Hydatid Disease 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors received financial support from Shiraz University (grant No. 87-GR-VT-24). We acknowledge Mr. A. Motabi Alavi for his kind assistance during this study.

Supplementary material

(WMV 16,098 kb)

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Copyright information

© Société Internationale de Chirurgie 2010

Authors and Affiliations

  1. 1.Department of PathobiologyShiraz UniversityShirazIran

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