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Aesthetic Plastic Surgery

, Volume 43, Issue 5, pp 1353–1361 | Cite as

A Reliable Stem Cell Carrier: An Experimental Study in Wistar Rats

  • Ithamar Nogueira StoccheroEmail author
  • Nelson Foresto Lizier
  • Rafael Fantelli Stelini
  • Olívia Capela Grimaldi de Oliveira
  • Paulo Roberto Grimaldi de Oliveira
  • Carlos Alexandre Ayoub
  • Tainah Desuó Rotta
  • Guilherme Flosi Stocchero
  • Paulo Kharmandayan
Original Article Basic Science/Experimental
  • 109 Downloads

Abstract

Introduction

Treatments based on cell biology need reliable and precise carriers for reaching the desired targets. For that reason, a PDO-based cell carrier was idealized, with the purpose of carrying stem cells to distant sites at room temperature.

Materials and Methods

Three modalities of the same carrier were evaluated: one containing undifferentiated human dental pulp stem cells (DPSCs); one loaded with stem cells induced to neurogenic differentiation (DPSCNs); and one without cells (Blank). The carriers were implanted in sciatic nerve gaps in 48 Wistar rats that were divided in three groups. Two other rats were included in a SHAM control group. Immunohistochemical, histological and clinical analyses were performed in two, four, six and eight weeks of time.

Results

Efficacy of human stem cell transportation at room temperature to rats was attested. Moreover, it was possible to confirm that those cells show tropism for inflamed environments and are also prone to induction of neurogenesis in the first two weeks, vanishing after that period.

Conclusion

Clinical evaluation of the animals’ gait recovery shows a promising perspective of success with the inclusion of stem cell-loaded PDO tubes in nerve gaps, which may be positively compared to previously published studies.

No level assigned

This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors - www.springer.com/00266.

Keywords

Stem cells PDO carrier Nerve regeneration Nerve guide Tropism for inflammation 

Notes

Acknowledgements

The authors are grateful to Engineer Erich Odermatt for the materialization of the idea about the carrier; to Aesculap/B. Braun for the production of the carrier, its supply and the suture threads; to pharmacist-biochemist Cristina Tanikawa for supplying the two SHAM animals; to Laboratório Pathos Daignósticos Médicos for performing the immunohistochemical analyses; to William Adalberto Silva, biologist, and Waldemir Benedito Costa and Miguel Luís Cândido, animal laboratory technicians. They gave all the support needed for the success in the animal research.

Financial Support

Acquisition of the rats and biological products (such as antibodies, antibiotics and analgesics) used in the study was funded by the senior author.

Compliance with Ethical Standards

Disclosure

The main author has an Intellectual Property (Patent) of a biological carrier since 2008, in the USA. In August 2010, he signed a Collaboration Agreement with Aesculap/B. Braun in a potential production of the device. That is all to disclose.

Statement

All applicable institutional and national guidelines for the care and use of animals were followed.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature and International Society of Aesthetic Plastic Surgery 2019

Authors and Affiliations

  • Ithamar Nogueira Stocchero
    • 1
    • 2
    Email author
  • Nelson Foresto Lizier
    • 3
  • Rafael Fantelli Stelini
    • 4
  • Olívia Capela Grimaldi de Oliveira
    • 5
  • Paulo Roberto Grimaldi de Oliveira
    • 6
  • Carlos Alexandre Ayoub
    • 7
  • Tainah Desuó Rotta
    • 4
  • Guilherme Flosi Stocchero
    • 8
  • Paulo Kharmandayan
    • 9
  1. 1.State University of Campinas Medical Sciences School – UNICAMPCampinasBrazil
  2. 2.São PauloBrazil
  3. 3.Biotechnologist Centro de Criogenia BrasilSão PauloBrazil
  4. 4.Department of Pathologic AnatomyUNICAMPCampinasBrazil
  5. 5.Pathos Diagnósticos MédicosSão PauloBrazil
  6. 6.Centro Universitário São Camilo Medical SchoolSão PauloBrazil
  7. 7.Centro de Criogenia BrasilSão PauloBrazil
  8. 8.Centro Médico Viver MelhorSão PauloBrazil
  9. 9.Division of Plastic and Reconstructive SurgeryState University of Campinas Medical Sciences SchoolCampinasBrazil

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