Effect of the Stromal Vascular Fraction on Changes in Melanin Formation in B16 Cells Treated by IBMX
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To investigate the effect of the stromal vascular fraction (SVF) on changes in melanin formation and tyrosinase activity in B16 cells treated by 3-isobutyl-1 methylxanthine (IBMX) and to explore the mechanism of SVF-mediated inhibition of pigmentation.
We co-cultured extracted SVFs and B16 cells treated with IBMX in a certain proportion, and the marker molecule HMB-45 was detected by immunochemistry. Melanin content was determined by NaOH lysis. Activity of tyrosinase was measured by the DOPA oxidation method.
HMB-45 was commonly expressed in B16 cells induced by IBMX. After the addition of SVFs, the expression of HMB-45 decreased significantly and positively correlated with increases in SVFs. After the induction of B16 cells by IBMX, melanin content increased significantly. However, melanin decreased after SVF and B16 co-culturing; the effect was more substantial with the increase and decrease in SVFs, and the activity of tyrosinase decreased.
SVFs inhibit the production of melanin and reduce the activity of tyrosinase, possibly providing a new breakthrough for the treatment of pigment disorders.
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KeywordsStromal vascular fraction (SVF) Tyrosinase Melanin B16 cells IBMX
This work was supported by the Guangdong medical science and technology research fund project (No. B2018073).
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflicts of interest to disclose.
Human and Animal Rights
This article does not contain any studies with human participants performed by any of the authors. The animal care and experimental protocols were approved by the Animal Research Committee of Southern Medical University.
For this type of study, informed consent is not required.
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