Advertisement

Aesthetic Plastic Surgery

, Volume 43, Issue 5, pp 1381–1386 | Cite as

Effect of the Stromal Vascular Fraction on Changes in Melanin Formation in B16 Cells Treated by IBMX

  • Li-hong Peng
  • Qin LiEmail author
Original Article Basic Science/Experimental
  • 90 Downloads

Abstract

Objective

To investigate the effect of the stromal vascular fraction (SVF) on changes in melanin formation and tyrosinase activity in B16 cells treated by 3-isobutyl-1 methylxanthine (IBMX) and to explore the mechanism of SVF-mediated inhibition of pigmentation.

Methods

We co-cultured extracted SVFs and B16 cells treated with IBMX in a certain proportion, and the marker molecule HMB-45 was detected by immunochemistry. Melanin content was determined by NaOH lysis. Activity of tyrosinase was measured by the DOPA oxidation method.

Results

HMB-45 was commonly expressed in B16 cells induced by IBMX. After the addition of SVFs, the expression of HMB-45 decreased significantly and positively correlated with increases in SVFs. After the induction of B16 cells by IBMX, melanin content increased significantly. However, melanin decreased after SVF and B16 co-culturing; the effect was more substantial with the increase and decrease in SVFs, and the activity of tyrosinase decreased.

Conclusion

SVFs inhibit the production of melanin and reduce the activity of tyrosinase, possibly providing a new breakthrough for the treatment of pigment disorders.

No Level Assigned

This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.

Keywords

Stromal vascular fraction (SVF) Tyrosinase Melanin B16 cells IBMX 

Notes

Acknowledgements

This work was supported by the Guangdong medical science and technology research fund project (No. B2018073).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflicts of interest to disclose.

Human and Animal Rights

This article does not contain any studies with human participants performed by any of the authors. The animal care and experimental protocols were approved by the Animal Research Committee of Southern Medical University.

Informed Consent

For this type of study, informed consent is not required.

References

  1. 1.
    Son YO, Lee SA, Kim SS et al (2011) Acteoside inhibits melanogenesis in B16F10 cells through ERK activation and tyrosinase down-regulation. J Pharm Pharmacol 63(10):1309–1319CrossRefPubMedGoogle Scholar
  2. 2.
    Lee HD, Lee WH, Roh E et al (2011) Manassantin A inhibits cAMP-induced melanin production by down-regulating the gene expressions of MITF and tyrosinase in melanocytes. Exp Dermatol 20(9):761–763CrossRefPubMedGoogle Scholar
  3. 3.
    Shibahara S, Takeda K, Yasumoto K et al (2001) Microphthalmia-associated transcription factor (MITF): multiplicity in structure, function, and regulation. J Investig Dermatol Symp Proc 6(1):99–104CrossRefPubMedGoogle Scholar
  4. 4.
    Gimble JM, Bunnell BA, Chiu ES et al (2011) Concise review: Adipose-derived stromal vascular fraction cells and stem cells: let’s not get lost in translation. Stem Cells 29(5):749–754CrossRefPubMedGoogle Scholar
  5. 5.
    Zuk PA, Zhu M, Ashjian P et al (2002) Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell 13(12):4279–4295CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Hunt G, Todd C, Cresswell JE et al (1994) Alpha-melanocyte stimulating hormone and its analogue Nle4DPhe7 alpha-MSH affect morphology, tyrosinase activity and melanogenesis in cultured human melanocytes. J Cell Sci 107(1):205–211PubMedGoogle Scholar
  7. 7.
    Lu S, Slominski A, Yang SE, Sheehan C, Ross J, Carlson JA (2010) The correlation of TRPM1 (Melastatin) mRNA expression with microphthalmia-associated transcription factor (MITF) and other melanogenesis-related proteins in normal and pathological skin, hair follicles and melanocytic nevi. J Cutan Pathol 37:26–40CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Slominski A (2002) Coming of age of melanogenesis-related proteins. Arch Pathol Lab Med 126(7):775–777PubMedGoogle Scholar
  9. 9.
    Carlson JA, Ross JS, Slominski AJ (2009) New techniques in dermatopathology that help to diagnose and prognosticate melanoma. Clin Dermatol 27(1):75–102CrossRefPubMedGoogle Scholar
  10. 10.
    Busam KJ (2004) The use and application of special techniques in assessing melanocytic tumours. Pathology 36(5):462–469CrossRefPubMedGoogle Scholar
  11. 11.
    Imokawa G (2004) Autocrine and paracrine regulation of melanocytes in human skin and in pigmentary disorders. Pigment Cell Res 17(2):96–110CrossRefPubMedGoogle Scholar
  12. 12.
    Bennett DC, Lamoreux ML (2003) The color loci of mice–a genetic century. Pigment Cell Res 16(4):333–344CrossRefPubMedGoogle Scholar
  13. 13.
    Quevedo WC Jr, Holstein TJ, Dyckman J, McDonald CJ, Isaacson EL (2000) Inhibition of UVR-Induced Tanning and Immunosuppression by Topical Applications of Vitamins C and E to the Skin of Hairless (hr/hr) Mice 1. Pigment Cell Res 13(2):89–98CrossRefPubMedGoogle Scholar
  14. 14.
    Arora P, Sarkar R, Garg VK et al (2012) Lasers for treatment of melasma and post-inflammatory hyperpigmentation. J Cutan Aesthet Surg 5(2):93–103CrossRefPubMedPubMedCentralGoogle Scholar
  15. 15.
    Alexis AF (2013) Lasers and light-based therapies in ethnic skin: treatment options and recommendations for Fitzpatrick skin types V and VI. Br J Dermatol 169:91–97CrossRefPubMedGoogle Scholar
  16. 16.
    Denning MF (2012) Specifying protein kinase C functions in melanoma. Pigment Cell Melanoma Res 25(4):466–476CrossRefPubMedGoogle Scholar
  17. 17.
    Park HY, Wu C, Yonemoto L et al (2006) MITF mediates cAMP-induced protein kinase C-beta expression in human melanocytes. Biochem J 395(3):571–578CrossRefPubMedPubMedCentralGoogle Scholar
  18. 18.
    Bae-Harboe YS, Park HY (2012) Tyrosinase: a central regulatory protein for cutaneous pigmentation. J Invest Dermatol 132(12):2678–2680CrossRefPubMedGoogle Scholar
  19. 19.
    Kim WS, Park SH, Ahn SJ et al (2008) Whitening effect of adipose-derived stem cells: a critical role of TGF-beta 1. Biol Pharm Bull 31(4):606–610CrossRefPubMedGoogle Scholar
  20. 20.
    Chang H, Park JH, Min KH et al (2014) Whitening effects of adipose-derived stem cells: a preliminary in vivo study. Aesthetic Plast Surg 38(1):230–233CrossRefPubMedGoogle Scholar
  21. 21.
    Bunnell BA, Flaat M, Gagliardi C et al (2008) Adipose-derived stem cells: isolation, expansion and differentiation. Methods 45(2):115–120CrossRefPubMedPubMedCentralGoogle Scholar
  22. 22.
    Nürnberger S, Lindner C, Maier J, Strohmeier K, Wurzer C, Slezak P, Suessner S et al (2019) Adipose-tissue-derived therapeutic cells in their natural environment as an autologous cell therapy strategy: the microtissue-stromal vascular fraction. Eur Cells Mater 37:113–133CrossRefGoogle Scholar
  23. 23.
    van Dongen JA, Stevens HP, Parvizi M, van der Lei B, Harmsen MC (2016) The fractionation of adipose tissue procedure to obtain stromal vascular fractions for regenerative purposes. Wound Repair Regen 24(6):994–1003CrossRefPubMedGoogle Scholar
  24. 24.
    Chaowattanapanit S, Silpa-Archa N, Kohli I et al (2017) Postinflammatory hyperpigmentation: A comprehensive overview: Treatment options and prevention. J Am Acad Dermatol 77(4):607–621CrossRefPubMedGoogle Scholar
  25. 25.
    Kokai LE, Marra K, Rubin JP (2014) Adipose stem cells: biology and clinical applications for tissue repair and regeneration. Trans Res J Lab Clin Med 163(4):399–408Google Scholar
  26. 26.
    Rigotti G, Marchi A, Galie M, Baroni G, Benati D, Krampera M, Pasini A, Sbarbati A (2007) Clinical treatment of radiotherapy tissue damage by lipoaspirate transplant: a healing process mediated by adipose-derived adult stem cells. Plast Reconstr Surg 119(5):1409–1422 discussion 23–24 CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature and International Society of Aesthetic Plastic Surgery 2019

Authors and Affiliations

  1. 1.Southern Medical UniversityGuangzhouChina
  2. 2.Department of Plastic Surgery and Burn Center, Second Affiliated HospitalShantou University Medical CollegeShantouChina
  3. 3.Guangzhou School of Clinical MedicineSouthern Medical University (Guangzhou General Hospital of Guangzhou Military Region)GuangzhouChina

Personalised recommendations