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Effects of Platelet-Rich Plasma on Fat and Nanofat Survival: An Experimental Study on Mice

  • Xiaoxuan Lei
  • Huan Liu
  • Mengru Pang
  • Zhifang Zheng
  • Xi Tan
  • Biao ChengEmail author
Original Article Basic Science/Experimental
  • 69 Downloads

Abstract

Background

Nanofat and fat graft survival is an important clinical problem. The authors of this study investigated whether PRP has an impact on fat and nanofat graft survival and vascularization in a mouse model.

Materials and Methods

Fat was harvested from a 50-year-old healthy woman by vacuum suction, and nanofat was obtained by emulsification and centrifugation procedures. PRP was collected after two rounds of centrifugation from an autologous blood sample. Twenty male nude mice were divided into four treatment groups: PRP/nanofat, PRP/fat, saline/nanofat and saline/fat. After 1 month and 3 months, the grafts were extracted and weighed. The microstructure of the fat and nanofat was examined with a scanning electron microscope. HE and immunohistochemical staining was applied to observe neovascularization. Western blot analysis was used to analyse the expression of CD31 and VEGF.

Results

In fat tissue, fat cells had normal connections; the fat structure was complete and fibre networks were visible. In nanofat, the extracellular matrix vascular components were visible and their structures were intact. At 1 month and 3 months, the graft weights in the PRP/fat group were significantly higher than those in the other groups. Further, a higher degree of neovascularization was observed in the PRP/nanofat group, and the expression of CD31 and VEGF in the PRP/nanofat group was higher than that in the other groups.

Conclusion

PRP can promote nanofat and fat graft survival and vascularization.

No Level Assigned

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Keywords

Platelet-rich plasma Nanofat Fat graft Adipose-derived stem cells Nude mice 

Notes

Acknowledgements

This study was supported by the National Natural Science Foundation of China (Nos. 81171812, 81671924 and 81272105), the National Key Research and Development Plan of China (No. 2017YFC1103301), the National Basic Science and Development Program (No. 2012CB518105), Health and Medical Treatment Collaborative Innovation Major Special Projects of Guangzhou (No. 201508020253), the Science and Technology Program of Guangzhou (201508020115) and Science and Technology Project of Guangdong province (Nos. 2014B020212010, 508113150092, 2015A010101313 and 2017A050506011).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflicts of interest in relation to this work. We declare that we do not have any commercial or associative interest that represents a conflict of interest in connection with the work submitted.

Statement of Human and Animal Rights

All the experiments were approved by the Animal Care Committee of Guangzhou General Hospital. All applicable institutional and/or national guidelines for the care and use of animals were followed. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee.

Informed Consent

A 50-year-old healthy woman was selected for abdominal liposuction from the Plastic Surgery Department of the General Hospital. Her signed informed consent was obtained before the procedure was performed.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature and International Society of Aesthetic Plastic Surgery 2019

Authors and Affiliations

  • Xiaoxuan Lei
    • 1
    • 2
  • Huan Liu
    • 1
    • 2
  • Mengru Pang
    • 1
    • 2
  • Zhifang Zheng
    • 1
  • Xi Tan
    • 2
  • Biao Cheng
    • 1
    • 2
    Email author
  1. 1.The Graduate School of Southern Medical UniversityGuangzhouChina
  2. 2.Center of Wound TreatmentGeneral Hospital of Southern Theater Command, PLAGuangzhouChina

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