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Discussion: Clinical Impact of Highly Condensed Stromal Vascular Fraction Injection in Surgical Management of Depressed and Contracted Scars

  • Seong Joo Lee
  • In Suck Suh
  • Hii Sun JeongEmail author
Editor’s Invited Commentary
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Level of Evidence V This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.

Dear Editors,

We appreciate your comments on our paper, in which you suggested alternatives to the enzymatic stromal vascular fraction (SVF) and safety issues. We were very interested in your opinions.

Your first comment was on the usage of type II collagenase. This enzyme is used widely for the enzymatic digestion of centrifuged fat, as it dissociates adipose cell membranes most efficiently. However, to extract stem cells from adipose tissue, it is more useful to digest the collagen rather than the adipose cell membrane. As the substrates of type II collagenase are collagen types I, III, V, VII, VIII, and X, fibronectin, gelatin, and aggrecan, this enzyme has a better harvesting effect on cells from dermal and fat tissue....

Notes

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

Informed consent

Additional informed consent was obtained from all individual participants for whom identifying information is included in this article.

References

  1. 1.
    Lee HY, Yang HJ (2015) Integrated technology of stem cells therapy; MMCC (minimally manipulating closed culture). Medikan, SeoulGoogle Scholar
  2. 2.
    McCarthy RC, Breite AG, Green ML, Dwulet FE (2011) Tissue dissociation enzymes for isolating human islets for transplantation: factors to consider in setting enzyme acceptance criteria. Transplantation 91:137–145CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Pu LL, Coleman SR, Cui X, Ferguson RE Jr, Vasconez HC (2008) Autologous fat grafts harvested and refined by the Coleman technique: a comparative study. Plast Reconstr Surg 122:932–937CrossRefPubMedGoogle Scholar
  4. 4.
    Strong AL, Cederna PS, Rubin JP, Coleman SR, Levi B (2015) The current state of fat grafting: a review of harvesting, processing, and injection techniques. Plast Reconstr Surg 136:897–912CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Yao Y, Dong Z, Liao Y et al (2017) Adipose extracellular matrix/stromal vascular fraction gel: a novel adipose tissue-derived injectable for stem cell therapy. Plast Reconstr Surg 139:867–879CrossRefPubMedGoogle Scholar
  6. 6.
    Chaput B, Bertheuil N, Escubes M et al (2016) Mechanically isolated stromal vascular fraction provides a valid and useful collagenase-free alternative technique: a comparative study. Plast Reconstr Surg 138:807–819CrossRefPubMedGoogle Scholar
  7. 7.
    Chang H, Do BR, Che JH et al (2013) Safety of adipose-derived stem cells and collagenase in fat tissue preparation. Aesthetic Plast Surg 37:802–808CrossRefPubMedGoogle Scholar
  8. 8.
    Aronowitz JA, Lockhart RA, Hakakian CS (2015) Mechanical versus enzymatic isolation of stromal vascular fraction cells from adipose tissue. Springerplus 4:713CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Shi L, Carson D (2009) Collagenase Santyl ointment: a selective agent for wound debridement. J Wound Ostomy Continence Nurs 36:S12–S16CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature and International Society of Aesthetic Plastic Surgery 2019

Authors and Affiliations

  1. 1.Department of Plastic and Reconstructive Surgery, Kangnam Sacred Heart Hospital, College of MedicineHallym UniversitySeoulKorea

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