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Aesthetic Plastic Surgery

, Volume 43, Issue 2, pp 514–520 | Cite as

M1/M2 Macrophages Play Different Roles in Adipogenic Differentiation of PDGFRα+ Preadipocytes In Vitro

  • Hao Cheng
  • Jie Luan
  • Dali Mu
  • Qian Wang
  • Jun Qi
  • Zifei Li
  • Su FuEmail author
Original Article Basic Science/Experimental
  • 71 Downloads

Abstract

Background

PGGFRα+ preadipocytes are the major subpopulations that can regenerate into adipocytes. Two different types of macrophages exist in the fat tissue: the classically activated macrophage (M1) and the alternatively activated macrophage (M2). In this study, we investigated whether M1/M2 macrophages play distinct roles in adipogenic differentiation of PDGFRα+ preadipocytes.

Methods

Mouse preadipocytes and macrophages were isolated from C57BL/6 male mice of 6–8 weeks. The culture supernate of M1 and M2 macrophages was collected and co-cultured with the PDGFRα+ preadipocytes. After 3 days, Oil Red O staining was used to evaluate to adipogenic differentiation of PDGFRα+ preadipocytes and the expression of adipogenic-related transcription factors (C/EBP-α, PPARγ) were also tested.

Results

The results showed that when cultured in vitro, M1 macrophages could significantly suppress the adipogenesis of PDGFRα+ preadipocytes as well as the C/EBP-α and PPARγ expression, but M2 macrophages did not have significant influence on the adipogenesis of PDGFRα+ preadipocytes nor on C/EBP-α and PPARγ expression compared with the control group.

Conclusions

M1 macrophages significantly suppress PDGFRα+ preadipocyte adipogenesis which provides a possible way to improve adipogenesis and fat retention after fat-free grafting by mitigating acute inflammation and manipulating M1 macrophage levels.

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Keywords

Macrophage Preadipocyte Adipogenic differentiation Adipogenesis 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 81501686) and CAMS Initiative for Innovative Medicine (CAMS-I2M) (2017-I2M-3-006).

Author’s Contribution

SF, JL, DLM and HC conceived and designed the experiments; HC performed the experiments, interpreted the data and prepared the figures; HC wrote the manuscript; ZFL, JQ and QW helped perform the experiments; SF and JL helped in writing the manuscript. All authors read and approved the final manuscript.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflicts of interest to disclose.

Human and Animal Rights

All applicable institutional and/or national guidelines for the care and use of animal were followed.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature and International Society of Aesthetic Plastic Surgery 2018

Authors and Affiliations

  • Hao Cheng
    • 1
  • Jie Luan
    • 1
  • Dali Mu
    • 1
  • Qian Wang
    • 2
  • Jun Qi
    • 1
  • Zifei Li
    • 1
  • Su Fu
    • 1
    Email author
  1. 1.Breast Plastic and Reconstructive Center, Plastic Surgery HospitalChinese Academy of Medical Sciences & Peking Union Medical CollegeBeijingPeople’s Republic of China
  2. 2.Research Center of Plastic Surgery HospitalChinese Academy of Medical Sciences & Peking Union Medical CollegeBeijingPeople’s Republic of China

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