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Aesthetic Plastic Surgery

, Volume 43, Issue 1, pp 228–232 | Cite as

Effect of Decantation Time on Viability and Apoptosis in Adipocytes After Liposuction

  • Gabriel A. MecottEmail author
  • Iram Z. Gonzalez
  • Roberto Montes de Oca
  • Rodolfo Garza-Morales
  • Ivan Gonzalez-Cantu
  • Yanko Castro-Govea
  • Odilia Saucedo-Cárdenas
  • Mauricio M. García-Pérez
Original Article Basic Science/Experimental
  • 129 Downloads

Abstract

Background

The effect of decantation time on viability and apoptosis in adipocytes has not been described. The objective of the study was to describe viability and apoptosis in adipocytes up to 2 h after harvesting.

Methods

Twenty patients who underwent esthetic liposuction from the abdomen were included. The lipoaspirate was obtained from the infra-umbilical area with the tumescent technique. Liposuction was performed with a 60-ml syringe and a 3-ml cannula. Lipoaspirates were centrifuged at 50 g for 5 min at 0, 60 and 120 min after harvesting. One gram of fat was digested with 0.1% type 1 collagenase and incubated at 37 degrees for 30 min. Adipocytes were counted on 10 random microscopic fields. Apoptosis was determined by TUNEL assay. A fluorescence microscope was used to visualize the staining nuclei and cells.

Results

Regarding viability, immediately after harvesting, 57.6 ± 18.9% of the cells were viable, whereas 60 min after liposuction the viability decreased to 51.62 ± 8.8% and 120 min after liposuction the percentage of viable cells was 46.8 ± 16.9%. The percentage of apoptotic cells at time 0 was 38.2 ± 8.0%, whereas it was 51.24 ± 8.1% at 60 min and 62.9 ± 16.1% at 120 min after collection.

Conclusions

Apoptosis and mortality of adipocytes after liposuction increase directly proportional to the time of decantation. Lipoinjection should be performed as soon as possible after harvesting.

No Level Assigned

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Keywords

Adipocytes Apoptosis Viability Decantation Fat grafting 

Notes

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature and International Society of Aesthetic Plastic Surgery 2018

Authors and Affiliations

  • Gabriel A. Mecott
    • 1
    Email author
  • Iram Z. Gonzalez
    • 1
  • Roberto Montes de Oca
    • 2
  • Rodolfo Garza-Morales
    • 2
  • Ivan Gonzalez-Cantu
    • 1
  • Yanko Castro-Govea
    • 1
  • Odilia Saucedo-Cárdenas
    • 2
  • Mauricio M. García-Pérez
    • 1
  1. 1.Department of Plastic SurgeryUniversidad Autonoma de Nuevo LeonMonterreyMexico
  2. 2.Histology Department, Faculty of MedicineUniversidad Autonoma de Nuevo LeonMonterreyMexico

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