Effect of Decantation Time on Viability and Apoptosis in Adipocytes After Liposuction
- 129 Downloads
The effect of decantation time on viability and apoptosis in adipocytes has not been described. The objective of the study was to describe viability and apoptosis in adipocytes up to 2 h after harvesting.
Twenty patients who underwent esthetic liposuction from the abdomen were included. The lipoaspirate was obtained from the infra-umbilical area with the tumescent technique. Liposuction was performed with a 60-ml syringe and a 3-ml cannula. Lipoaspirates were centrifuged at 50 g for 5 min at 0, 60 and 120 min after harvesting. One gram of fat was digested with 0.1% type 1 collagenase and incubated at 37 degrees for 30 min. Adipocytes were counted on 10 random microscopic fields. Apoptosis was determined by TUNEL assay. A fluorescence microscope was used to visualize the staining nuclei and cells.
Regarding viability, immediately after harvesting, 57.6 ± 18.9% of the cells were viable, whereas 60 min after liposuction the viability decreased to 51.62 ± 8.8% and 120 min after liposuction the percentage of viable cells was 46.8 ± 16.9%. The percentage of apoptotic cells at time 0 was 38.2 ± 8.0%, whereas it was 51.24 ± 8.1% at 60 min and 62.9 ± 16.1% at 120 min after collection.
Apoptosis and mortality of adipocytes after liposuction increase directly proportional to the time of decantation. Lipoinjection should be performed as soon as possible after harvesting.
No Level Assigned
This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.
KeywordsAdipocytes Apoptosis Viability Decantation Fat grafting
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interest.
- 3.Guerrerosantos J (1996) Autologous fat grafting for body contouring. Clin Plast Surg 23:619–631Google Scholar
- 4.Coleman SR (2004) Problems, complications and postprocedure care. In: Coleman SR (ed) Structural fat grafting. Quality Medical Publishing, St. Louis, pp 75–102Google Scholar
- 11.Coleman SR (1997) Facial recontouring with lipostructure. Clin Plast Surg 24:347–367Google Scholar
- 18.Neuber GA (1893) Fettransplantation. In: Paper presented at: Chir Kongr Verhandl Deutsche GEsellshaft fur ChirgurgieGoogle Scholar
- 19.Illouz YG (1990) Fat injection: a four-year clinical trial. In: Hetter GP (ed) Lipoplasty: the theory and practice of blunt suction lipectomy, 2nd edn. Little, Brown, Boston, pp 239–246Google Scholar