Raman spectroscopy reveals differences in molecular structure between human femoral heads affected by steroid-associated and alcohol-associated osteonecrosis

  • Ema Nakahara
  • Wenliang Zhu
  • Giuseppe Pezzotti
  • Hidetoshi Hamada
  • Masaki Takao
  • Takashi Sakai
  • Nobuhiko Sugano
Original Paper

Abstract

Purpose

The purposes of this study were to document novel Raman spectroscopic findings in femoral heads affected by osteonecrosis and to identify molecular structure differences based on aetiology.

Methods

We obtained 13 femoral heads with osteonecrosis from 13 different patients who underwent total hip arthroplasty. Comparisons were made between the viable zones of each femoral head examined. The samples were scanned with X-ray micro-CT for structural mapping and a central coronal section slab was prepared for Raman spectroscopy and histological analyses. Raman spectra were collected at different locations, including the viable and necrotic zones of the femoral head, using a highly spectrally resolved Raman microprobe.

Results

Significant alterations in the spectral morphology in the high wavenumber region were found, with a pronounced inhibition of peculiar lipid signals in the frequency interval 2851 ~ 2890 cm−1 and at ~ 1750 cm−1. The necrotic zone in steroid-associated osteonecrosis showed an increase in the ratio of lipid-related bands to protein-related bands, while alcohol-associated osteonecrosis exhibited a decrease in this ratio.

Conclusions

We systematically found a decrease in Raman intensity for sphingomyelin and phenylalanine fingerprint bands in the necrotic zones, and these differences may be related to the etiology of osteonecrosis.

Keywords

Osteonecrosis of the femoral head Raman Spectroscopy Aetiologic factor Histology 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© SICOT aisbl 2018
corrected publication April/2018

Authors and Affiliations

  • Ema Nakahara
    • 1
  • Wenliang Zhu
    • 1
  • Giuseppe Pezzotti
    • 2
  • Hidetoshi Hamada
    • 3
  • Masaki Takao
    • 1
  • Takashi Sakai
    • 3
  • Nobuhiko Sugano
    • 1
  1. 1.Department of Orthopaedic Medical EngineeringOsaka University Graduate School of MedicineOsakaJapan
  2. 2.Ceramic Physics LaboratoryKyoto Institute of TechnologyKyotoJapan
  3. 3.Department of Orthopaedic SurgeryOsaka University Graduate School of MedicineOsakaJapan

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