Cellular reactions to biodegradable magnesium alloys on human growth plate chondrocytes and osteoblasts
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In recent decades operative fracture treatment using elastic stable intramedullary nails (ESINs) has mainly taken precedence over conservative alternatives in children. The development of biodegradable materials that could be used for ESINs would be a further step towards treatment improvement. Due to its mechanical and elastic properties, magnesium seems to be an ideal material for biodegradable implant application. The aim of this study was therefore to investigate the cellular reaction to biodegradable magnesium implants in vitro.
Primary human growth plate chondrocytes and MG63 osteoblasts were used for this study. Viability and metabolic activity in response to the eluate of a rapidly and a slower degrading magnesium alloy were investigated. Furthermore, changes in gene expression were assessed and live cell imaging was performed.
A superior performance of the slower degrading WZ21 alloy’s eluate was detected regarding cell viability and metabolic activity, cell proliferation and morphology. However, the ZX50 alloy’s eluate induced a favourable up-regulation of osteogenic markers in MG63 osteoblasts.
This study showed that magnesium alloys for use in biodegradable implant application are well tolerated in both osteoblasts and growth plate chondrocytes respectively.
KeywordsBiodegradable magnesium Orthopaedics Immature skeleton Growth Biocompatibility
The authors appreciate support from the Laura Bassi Center of Expertise BRIC (Bioresorbable Implants for Children; FFG – Austria) and from the Staub/Kaiser Foundation, Switzerland. Furthermore, they would like to thank Mr. Rudolf Schmied for his valuable technical assistance in art work preparation and Ms. Aranka Schauer for her help in carrying out some of the experiments.
hGPC treated with the ZX50 eluate. (MPEG 2.66 MB)
hGPC treated with the WZ21 eluate. (MPEG 2.64 MB)
hGPC treated with SBF. (MPEG 2.35 MB)
Untreated hGPC. (MPEG 2.30 MB)
MG63 osteoblasts treated with the ZX50 eluate. (MPEG 2.70 MB)
MG63 osteoblasts treated with the WZ21 eluate. (MPEG 2.71 MB)
MG63 osteoblasts treated with SBF. (MPEG 2.71 MB)
Untreated MG63 osteoblasts. (MPEG 2.64 MB)
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