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Fabrication of biopolymer polyhydroxyalkanoate/chitosan and 2D molybdenum disulfide–doped scaffolds for antibacterial and biomedical applications

Abstract

Antibiotic resistance in pathogenic bacteria is a major health challenge, as Infectious Diseases Society of America (IDSA) has recognized that the past simply drugs susceptible pathogens are now the most dangerous pathogens due to their nonstop growing resistance towards conventional antibiotics. Therefore, due to the emergence of multi-drug resistance, the bacterial infections have become a serious global problem. Acute infections feasibly develop into chronic infections because of many factors; one of them is the failure of effectiveness of antibiotics against superbugs. Modern research of two-dimensional nanoparticles and biopolymers are of great interest to attain the intricate bactericidal activity. In this study, we fabricated an antibacterial nanocomposite consisting of representative two-dimensional molybdenum disulfide (2D MoS2) nanoparticles. Polyhydroxyalkanoate (PHA) and chitosan (Ch) are used to encapsulate MoS2 nanoparticles into their matrix. This study reports the in vitro antibacterial activity and host cytotoxicity of novel PHA-Ch/MoS2 nanocomposites. PHA-Ch/MoS2 nanocomposites were subjected to time-dependent antibacterial assays at various doses to examine their antibacterial activity against multi-drug-resistant Escherichia coli K1 (Malaysian Type Culture Collection 710859) and methicillin-resistant Staphylococcus aureus (MRSA) (Malaysian Type Culture Collection 381123). Furthermore, the cytotoxicity of nanocomposites was examined against spontaneously immortalized human keratinocyte (HaCaT) cell lines. The results indicated significant antibacterial activity (p value < 0.05) against E. coli K1 and MRSA. In addition, PHA-Ch/MoS2 showed significant host cytocompatibility (p < 0.05) against HaCaT cells. The fabricated PHA-Ch/MoS2 nanocomposites have demonstrated effective antibacterial activity against both Gram-positive and -negative bacteria and exhibited better biocompatibility. Finally, PHA-Ch/MoS2 nanocomposites are shown to be suitable for antibacterial applications and also hold potential for further biomedical studies.

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References

  1. Ahmed D, Anwar A, Khan AK, Ahmed A, Shah MR, Khan NA (2017) Size selectivity in antibiofilm activity of 3-(diphenylphosphino) propanoic acid coated gold nanomaterials against Gram-positive Staphylococcus aureus and Streptococcus mutans. AMB Express 7(1):210

  2. Anwar A, Khalid S, Perveen S, Ahmed S, Siddiqui R, Khan NA, Shah MR (2018) Synthesis of 4-(dimethylamino) pyridine propylthioacetate coated gold nanoparticles and their antibacterial and photophysical activity. J Nanobiotechnol 16(1):6

  3. Arabi Shamsabadi A, Sharifian Gh M, Anasori B, Soroush M (2018) Antimicrobial mode-of-action of colloidal Ti3C2Tx MXene nanosheets. ACS Sustain Chem Eng 6(12):16586–16596

  4. Balouiri M, Sadiki M, Ibnsouda SK (2016) Methods for in vitro evaluating antimicrobial activity: a review. J Pharm Anal 6(2):71–79

  5. Boucher HW, Talbot GH, Bradley JS, Edwards JE, Gilbert D, Rice LB, Scheld M, Spellberg B, Bartlett J (2009) Bad bugs, no drugs: no ESKAPE! An update from the Infectious Diseases Society of America. Clin Infect Dis 48(1):1–12

  6. Cavalcanti A, Shirinzadeh B, Freitas RA Jr, Hogg T (2007) Nanorobot architecture for medical target identification. Nanotechnology 19(1):015103

  7. Czaplewski L, Bax R, Clokie M, Dawson M, Fairhead H, Fischetti VA, Foster S, Gilmore BF, Hancock REW, Harper D, Henderson IR, Hilpert K, Jones BV, Kadioglu A, Knowles D, Ólafsdóttir S, Payne D, Projan S, Shaunak S, Silverman J, Thomas CM, Trust TJ, Warn P, Rex JH (2016) Alternatives to antibiotics—a pipeline portfolio review. The Lancet Infect Dis 16(2):239–251

  8. Elieh-Ali-Komi D, Hamblin MR (2016) Chitin and chitosan: production and application of versatile biomedical nanomaterials. Int J Adv Res 4(3):411–427

  9. Hajipour MJ, Fromm KM, Ashkarran AA, de Aberasturi DJ, de Larramendi IR, Rojo T, Serpooshan V, Parak WJ, Mahmoudi M (2012) Antibacterial properties of nanoparticles. Trends Biotechnol 30(10):499–511

  10. Kim TI, Kwon B, Yoon J, Park IJ, Bang GS, Park Y, Seo YS, Choi SY (2017) Antibacterial activities of graphene oxide–molybdenum disulfide nanocomposite films. ACS Appl Mater Interfaces 9(9):7908–7917

  11. Li Z, Yang J, Loh XJ (2016) Polyhydroxyalkanoates: opening doors for a sustainable future. NPG Asia Mater 8(4):e265

  12. Liu S, Zeng TH, Hofmann M, Burcombe E, Wei J, Jiang R, Kong J, Chen Y (2011) Antibacterial activity of graphite, graphite oxide, graphene oxide, and reduced graphene oxide: membrane and oxidative stress. ACS Nano 5(9):6971–6980

  13. Liu Y, Peng J, Wang S, Xu M, Gao M, Xia T, Weng J, Xu A, Liu S (2018) Molybdenum disulfide/graphene oxide nanocomposites show favorable lung targeting and enhanced drug loading/tumor-killing efficacy with improved biocompatibility. NPG Asia Mater 10(1):e458

  14. McKenna M (2013) The last resort. Nature 499(7459):394–396

  15. Mobasser S, Firoozi AA (2016) Review of nanotechnology applications in science and engineering. J Civil Eng Urban 6(4):84–93

  16. Mukheem A, Muthoosamy K, Manickam S, Sudesh K, Shahabuddin S, Saidur R, Akbar N, Sridewi N (2018) Fabrication and characterization of an electrospun pha/graphene silver nanocomposite scaffold for antibacterial applications. Materials 11(9):1673

  17. Mushtaq S, Khan JA, Rabbani F, Latif U, Arfan M, Yameen MA (2017) Biocompatible biodegradable polymeric antibacterial nanoparticles for enhancing the effects of a third-generation cephalosporin against resistant bacteria. J Med Microbiol 66(3):318–327

  18. Nasr M, Soussan L, Viter R, Eid C, Habchi R, Miele P, Bechelany M (2018) High photodegradation and antibacterial activity of BN–Ag/TiO 2 composite nanofibers under visible light. New J Chem 42(2):1250–1259

  19. Nithya JSM, Pandurangan A (2014) Aqueous dispersion of polymer coated boron nitride nanotubes and their antibacterial and cytotoxicity studies. RSC Adv 4(60):32031–32046

  20. Qi L, Xu Z, Jiang X, Hu C, Zou X (2004) Preparation and antibacterial activity of chitosan nanoparticles. Carbohydr Res 339(16):2693–2700

  21. Sagheer M, Siddiqui R, Iqbal J, Khan NA (2014) Black cobra (Naja naja karachiensis) lysates exhibit broad-spectrum antimicrobial activities. Pathog Glob Health 108(3):129–136

  22. Salim YS, Chan CH, Sudesh K, Gan SN Influence of thermal treatment on the molecular weights of polyhydroxyalkanoate containing 3-hydroxyhexanoate. In: Advanced Materials Research, 2013. vol 812. Trans Tech Publ, p 250–253

  23. Shahabuddin S, Sarih NM, Ismail FH, Shahid MM, Huang NM (2015) Synthesis of chitosan grafted-polyaniline/Co 3 O 4 nanocube nanocomposites and their photocatalytic activity toward methylene blue dye degradation. RSC Adv 5(102):83857–83867

  24. Shih W-J, Chen Y-H, Shih C-J, Hon M-H, Wang M-C (2007) Structural and morphological studies on poly (3-hydroxybutyrate acid)(PHB)/chitosan drug releasing microspheres prepared by both single and double emulsion processes. J Alloys Compd 434:826–829

  25. Shrivastav A, Kim H-Y, Kim Y-R (2013) Advances in the applications of polyhydroxyalkanoate nanoparticles for novel drug delivery system. Biomed Res Int 2013:581684

  26. Spellberg B, Guidos R, Gilbert D, Bradley J, Boucher HW, Scheld WM, Bartlett JG, Edwards J Jr, America IDSo (2008) The epidemic of antibiotic-resistant infections: a call to action for the medical community from the Infectious Diseases Society of America. Clin Infect Dis 46(2):155–164

  27. Steinbüchel A, Lütke-Eversloh T (2003) Metabolic engineering and pathway construction for biotechnological production of relevant polyhydroxyalkanoates in microorganisms. Biochem Eng J 16(2):81–96

  28. Valappil SP, Misra SK, Boccaccini AR, Roy I (2006) Biomedical applications of polyhydroxyalkanoates, an overview of animal testing and in vivo responses. Expert Rev Med Devices 3(6):853–868

  29. Vattikuti S, Byon C (2015) Synthesis and characterization of molybdenum disulfide nanoflowers and nanosheets: nanotribology. J Nanomater 2015:9

  30. Ventola CL (2015) The antibiotic resistance crisis: part 1: causes and threats. Pharm Ther 40(4):277

  31. Vunain E, Mishra A, Mamba B (2017) Fundamentals of chitosan for biomedical applications Chitosan Based Biomaterials Volume 1. Elsevier, pp 3-30

  32. Walsh CT, Wencewicz TA (2014) Prospects for new antibiotics: a molecule-centered perspective. J Antibiotics 67(1):7

  33. Wu M-C, Deokar AR, Liao J-H, Shih P-Y, Ling Y-C (2013) Graphene-based photothermal agent for rapid and effective killing of bacteria. ACS Nano 7(2):1281–1290

  34. Wu N, Yu Y, Li T, Ji X, Jiang L, Zong J, Huang H (2016) Investigating the influence of mos2 nanosheets on E. coli from metabolomics level. PLoS One 11(12):e0167245

  35. Yang X, Li J, Liang T, Ma C, Zhang Y, Chen H, Hanagata N, Su H, Xu M (2014) Antibacterial activity of two-dimensional MoS 2 sheets. Nanoscale 6(17):10126–10133

  36. Zhang W, Shi S, Wang Y, Yu S, Zhu W, Zhang X, Zhang D, Yang B, Wang X, Wang J (2016) Versatile molybdenum disulfide based antibacterial composites for in vitro enhanced sterilization and in vivo focal infection therapy. Nanoscale 8(22):11642–11648

  37. Zinn M, Witholt B, Egli T (2001) Occurrence, synthesis and medical application of bacterial polyhydroxyalkanoate. Adv Drug Deliv Rev 53(1):5–21

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Funding

The authors would like to thank the Ministry of Science, Technology and Innovation (MOSTI), Malaysia (No. 2014/03-01-19-SF0127) and Pandit Deendayal Petroleum University for providing the research support.

Author information

AM and SS synthesized the materials. NMS and KS characterized the materials. NA, AA, and NAK performed the biological studies. SS and NS supervised the study. AM and SS wrote the manuscript. The draft was finalized by SS and NS. All authors read and approved the final manuscript.

Correspondence to Syed Shahabuddin or Ayaz Anwar or Nanthini Sridewi.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Mukheem, A., Shahabuddin, S., Akbar, N. et al. Fabrication of biopolymer polyhydroxyalkanoate/chitosan and 2D molybdenum disulfide–doped scaffolds for antibacterial and biomedical applications. Appl Microbiol Biotechnol (2020). https://doi.org/10.1007/s00253-020-10416-2

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Keywords

  • Molybdenum disulfide
  • Chitosan
  • Polyhydroxyalkanoate
  • Antibacterial
  • Biocompatible