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Sulfur-containing amino acid supplementation to gilts from late pregnancy to lactation altered offspring’s intestinal microbiota and plasma metabolites

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Abstract

Maternal nutrition during late pregnancy and lactation is highly involved with the offspring’s health status. The study was carried out to evaluate the effects of different ratios of methionine and cysteine (Met/Cys: 46% Met, 51% Met, 56% Met, and 62% Met; maintained with 0.78% of total sulfur-containing amino acids; details in “Materials and methods”) supplements in the sows’ diet from late pregnancy to lactation on offspring’s plasma metabolomics and intestinal microbiota. The results revealed that the level of serum albumin, calcium, iron, and magnesium was increased in the 51% Met group compared with the 46% Met, 56% Met, and 62% Met groups. Plasma metabolomics results indicated that the higher ratios of methionine and cysteine (0.51% Met, 0.56% Met, and 0.62% Met)–supplemented groups enriched the level of hippuric acid, retinoic acid, riboflavin, and δ-tocopherol than in the 46% Met group. Furthermore, the 51% Met–supplemented group had a higher relative abundance of Firmicutes compared with the other three groups (P < 0.05), while the 62% Met–supplemented group increased the abundance of Proteobacteria compared with the other three groups (P < 0.05) in piglets’ intestine. These results indicated that a diet consisting with 51% Met is the optimum Met/Cys ratio from late pregnancy to lactation can maintain the offspring’s health by improving the serum biochemical indicators and altering the plasma metabolomics profile and intestinal gut microbiota composition, but higher proportion of Met/Cys may increase the possible risk to offspring’s health.

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Acknowledgments

The authors acknowledge the CAS-TWAS President’s Fellowship for international PhD students.

Funding information

This study was supported by National Natural Science Foundation of China (No. 31772642, 31672457), International Partnership Program of Chinese Academy of Sciences (161343KYSB20160008), Hunan Provincial Science and Technology Department (2017NK2322, 2018CT5002, 2018WK4025), Local Science and Technology Development Project guided by The Central Government (YDZX20184300002303), Double first-class construction project of Hunan Agricultural University (SYL201802003, YB2018007, CX20190497), Project for Yunnan Yin Yulong Academician Workstation from Yunan Province (2018IC087), and China Postdoctoral Science Foundation (2018 M632963, 2019 T120705).

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Correspondence to Gang Liu.

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The study was carried out in accordance with the guidelines of the Laboratory Animal Ethical Commission of the Chinese Academy of Science and approved by the Animal Care Committee of the Institute of Subtropical Agriculture (201703-64C), Chinese Academy of Science, Changsha, China.

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Azad, M.A.K., Liu, G., Bin, P. et al. Sulfur-containing amino acid supplementation to gilts from late pregnancy to lactation altered offspring’s intestinal microbiota and plasma metabolites. Appl Microbiol Biotechnol 104, 1227–1242 (2020). https://doi.org/10.1007/s00253-019-10302-6

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Keywords

  • Sulfur-containing amino acids
  • Pregnancy
  • Lactation
  • Intestinal microbiota
  • Plasma metabolomics