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Applied Microbiology and Biotechnology

, Volume 104, Issue 3, pp 1283–1290 | Cite as

New approach for determination of antimicrobial susceptibility to antibiotics by an acoustic sensor

  • O. I. GuliyEmail author
  • B. D. Zaitsev
  • I. A. Borodina
Methods and protocols
  • 28 Downloads

Abstract

For the first time, a rapid method was proposed to determine the susceptibility of Escherichia coli cells to antibiotics by the example of ampicillin by using a biological sensor based on a slot mode in an acoustic delay line. It has been established that an indicator of the antibiotic activity to microbial cells is the difference between the recorded sensor’s signal before and after exposure cells with antibiotic. The depth and frequency of the peaks of resonant absorption in the frequency dependence of the insertion loss of sensor varied after adding an antibiotic with different concentrations to the microbial cells. By using the acoustic sensor based on slot-mode a criterion of E. coli sensitivity to ampicillin was established. The advantages of this method are the ability to carry out the analysis directly in the liquid, the short analysis time (within 10–15 min), and the possibility to reusable sensor.

Keywords

Escherichia coli Ampicillin Sensor based on a slot mode in acoustic delay line Express analysis of antibiotic susceptibility 

Notes

Funding information

This work was supported in part by the Russian Foundation for Basic Research (grant nos. 19-07-00300 and 19-07-00304).

Compliance with ethical standards

Ethical statement

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Conflict of interest

The author declares that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of Biochemistry and Physiology of Plants and MicroorganismsRussian Academy of SciencesSaratovRussia
  2. 2.Kotelnikov Institute of Radio Engineering and ElectronicsRussian Academy of SciencesSaratovRussia

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