Artificial transformation methodologies for improving the efficiency of plasmid DNA transformation and simplifying its use
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The uptake of exogenous DNA materials through the cell membrane by bacteria, known as transformation, is essential for the genetic manipulation of bacteria and, thus, plays key roles in biotechnological and biological research. The efficiency of natural transformation is very low; therefore, various artificial transformation methods have been developed for simple and efficient bacterial transformation. The basic bacterial transformation method is based on chemical, physical, and electrical processes and other means to permeabilize the bacterial cell membrane to allow plasmid DNA uptake. With the introduction of novel chemicals, materials, and devices and the optimization of protocols, new transformation methods have become simpler, cheaper, and more reproducible for use in diverse bacterial species compared with conventional methods. In this review, artificial transformation methods have been classified according to the membrane-permeabilizing mechanisms employed by them. Their influential factors, transformation efficiency, advantages, disadvantages, and practical applications are briefly illustrated. Finally, physicochemical transformation as a new bacterial transformation technique has also been described.
KeywordsBiotechnology Cell membrane permeability Genetic manipulation Plasmid DNA Transformation
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. NRF-2018R1A5A1025077). This work was also supported by an NRF grant funded by the Ministry of Science and ICT (2017R1A2B4004447).
Compliance with ethical standards
Conflict of interest
The authors declare that they no conflict of interest.
This work did not involve the direct study of humans or animals.
- Brito LF, Irla M, Walter T, Wendisch VF (2017) Magnesium aminoclay-based transformation of Paenibacillus riograndensis and Paenibacillus polymyxa and development of tools for gene expression. Appl Microbiol Biotechnol 101(2):735–747. https://doi.org/10.1007/s00253-016-7999-1 CrossRefPubMedGoogle Scholar
- Campos-Guillen J, Fernandez F, Pastrana X, Loske AM (2012) Relationship between plasmid size and shock wave-mediated bacterial transformation. Ultrasound Med Biol 38(6):1078–1084. https://doi.org/10.1016/j.ultrasmedbio.2012.02.018 CrossRefPubMedGoogle Scholar
- Chan WT, Verma CS, Lane DP, Gan SK (2013) A comparison and optimization of methods and factors affecting the transformation of Escherichia coli. Biosci Rep 33(6). https://doi.org/10.1042/BSR20130098
- Deshmukh K, Ramanan SR, Kowshik M (2019) Novel one step transformation method for Escherichia coli and Staphylococcus aureus using arginine-glucose functionalized hydroxyapatite nanoparticles. Mater Sci Eng C Mater Biol Appl 96:58–65. https://doi.org/10.1016/j.msec.2018.10.088 CrossRefPubMedGoogle Scholar
- Ding C, Pan J, Jin M, Yang D, Shen Z, Wang J, Zhang B, Liu W, Fu J, Guo X, Wang D, Chen Z, Yin J, Qiu Z, Li J (2016) Enhanced uptake of antibiotic resistance genes in the presence of nanoalumina. Nanotoxicology 10(8):1051–1060. https://doi.org/10.3109/17435390.2016.1161856 CrossRefPubMedGoogle Scholar
- Duitman EH, Wyczawski D, Boven LG, Venema G, Kuipers OP, Hamoen LW (2007) Novel methods for genetic transformation of natural Bacillus subtilis isolates used to study the regulation of the mycosubtilin and surfactin synthetases. Appl Environ Microbiol 73(11):3490–3496. https://doi.org/10.1128/AEM.02751-06 CrossRefPubMedPubMedCentralGoogle Scholar
- Kumari M, Pandey S, Mishra A, Nautiyal CS (2017) Finding a facile way for the bacterial DNA transformation by biosynthesized gold nanoparticles. FEMS Microbiol Lett 364(12). https://doi.org/10.1093/femsle/fnx081
- Yoshida N, Ikeda T, Yoshida T, Sengoku T, Ogawa K (2001) Chrysotile asbestos fibers mediate transformation of Escherichia coli by exogenous plasmid DNA. FEMS Microbiol Lett 195(2):133–137. https://doi.org/10.1111/j.1574-6968.2001.tb10510.x CrossRefPubMedGoogle Scholar
- Yoshida N, Kodama K, Nakata K, Yamashita M, Miwa T (2002) Escherichia coli cells penetrated by chrysotile fibers are transformed to antibiotic resistance by incorporation of exogenous plasmid DNA. Appl Microbiol Biotechnol 60(4):461–468. https://doi.org/10.1007/s00253-002-1148-8 CrossRefPubMedGoogle Scholar