Beneficial effect of a mixture of vitamin-producing and immune-modulating lactic acid bacteria as adjuvant for therapy in a recurrent mouse colitis model
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Inflammatory bowel diseases are chronic and relapsing-remitting disorders that affect the gastrointestinal tract. Previously, the administration of folate and riboflavin-producing lactic acid bacteria (LAB) or an immune-modulating strain showed beneficial effects as they were able to reduce the acute inflammation in mouse models. The aim of this work was to evaluate a mixture of vitamin-producing and immune-modulating LAB administering together with an anti-inflammatory drug during the remission period of a mouse model of recurrent colitis. BALB/c mice were intrarectally instilled with trinitrobenzene sulfonic acid (TNBS) and those who recovered from this acute challenge were given the LAB mixture, mesalazine, or the combination of both (mesalazine + LAB) during 21 days, followed by a second challenge with TNBS. Control mice instilled with ethanol (vehicle of TNBS) and receiving the different treatments were also evaluated in order to study the effect of chronic anti-inflammatory therapy. The combination of mesalazine and LAB mixture was the most effective to decrease the intestinal damage at macroscopic and histological levels and to reduce pro-inflammatory cytokines (IL-6 and TNF-α) in intestinal fluids. In animals instilled with ethanol, mesalazine produced a loss of body weight and intestinal damages with increased IL-6. These side effects were prevented by the co-administration of mesalazine and the LAB mixture. The LAB blend did not affect the primary anti-inflammatory treatment, was able to improve it, and also prevented the side effects of this therapy.
KeywordsFolate Riboflavin Immune modulation Anti-inflammatory therapy Recurrent colitis
This work was financially supported by CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas projects PIP006 and 0697) and ANPCyT (Agencia Nacional de Promoción Científica y Tecnológica projects 0301, 2554, 2859, and 3045).
Compliance with ethical standards
Conflict of interest
The authors declare that there are no conflicts of interest.
Animal experiments were approved by the Animal Protection Committee of CERELA and all experiments comply with the current laws of Argentina and international organizations for the use of experimental animals.
- Ameho CK, Adjei AA, Harrison EK, Takeshita K, Morioka T, Arakaki Y, Ito E, Suzuki I, Kulkarni AD, Kawajiri A (1997) Prophylactic effect of dietary glutamine supplementation on interleukin 8 and tumour necrosis factor α production in trinitrobenzene sulphonic acid induced colitis. Gut 41(4):487–493CrossRefGoogle Scholar
- Chae JM, Chang MH, Heo W, Cho HT, Lee DH, Hwang BB, Kim JW, Yoon SM, Yang S, Lee JH (2019) LB-9, novel probiotic lactic acid bacteria, ameliorates dextran sodium sulfate-induced colitis in mice by inhibiting TNF-α mediated apoptosis of intestinal epithelial cells. J Med Food 22(3):271–276CrossRefGoogle Scholar
- del Carmen S, de Moreno de LeBlanc A, Martin R, Chain F, Langella P, Bermúdez-Humarán LG, LeBlanc JG (2014) Genetically engineered immunomodulatory Streptococcus thermophilus strains producing antioxidant enzymes exhibit enhanced anti-inflammatory activities. App Environ Microbiol 80(3):869–877CrossRefGoogle Scholar
- Johnson AJ, Vangay P, Al-Ghalith GA, Hillmann BM, Ward TL, Shields-Cutler RR, Kim AD, Shmagel AK, Syed AN, Personalized Microbiome Class Students, Walter J, Menon R, Koecher K, Knights D (2019) Daily sampling reveals personalized diet-microbiome associations in humans. Cell Host Microbe 25(6):789–802CrossRefGoogle Scholar
- Levit R, Savoy de Giori G, de Moreno de LeBlanc A, LeBlanc JG (2018c) Increasing B vitamins in foods to prevent intestinal inflammation and cancer nutrients in dairy and their implications on health and disease. Elsevier:193–204Google Scholar
- Saez-Lara MJ, Gomez-Llorente C, Plaza-Diaz J, Gil A (2016) The role of probiotic lactic acid bacteria and bifidobacteria in the prevention and treatment of inflammatory bowel disease and other related diseases: a systematic review of randomized human clinical trials. Biomed Res Int 2015:1–15. https://doi.org/10.1155/2015/505878 CrossRefGoogle Scholar
- WHO FAO (2002) Probiotics in food health and nutritional properties and guidelines for evaluation. Food and Agriculture Organization of the United Nations and World Health Organization Expert Consultation Report. http://www.fao.org/3/a-a0512e.pdf. Accessed 29 July 2019