Beneficial effect of a mixture of vitamin-producing and immune-modulating lactic acid bacteria as adjuvant for therapy in a recurrent mouse colitis model

  • Romina Levit
  • Graciela Savoy de Giori
  • Alejandra de Moreno de LeBlancEmail author
  • Jean Guy LeBlancEmail author
Applied microbial and cell physiology


Inflammatory bowel diseases are chronic and relapsing-remitting disorders that affect the gastrointestinal tract. Previously, the administration of folate and riboflavin-producing lactic acid bacteria (LAB) or an immune-modulating strain showed beneficial effects as they were able to reduce the acute inflammation in mouse models. The aim of this work was to evaluate a mixture of vitamin-producing and immune-modulating LAB administering together with an anti-inflammatory drug during the remission period of a mouse model of recurrent colitis. BALB/c mice were intrarectally instilled with trinitrobenzene sulfonic acid (TNBS) and those who recovered from this acute challenge were given the LAB mixture, mesalazine, or the combination of both (mesalazine + LAB) during 21 days, followed by a second challenge with TNBS. Control mice instilled with ethanol (vehicle of TNBS) and receiving the different treatments were also evaluated in order to study the effect of chronic anti-inflammatory therapy. The combination of mesalazine and LAB mixture was the most effective to decrease the intestinal damage at macroscopic and histological levels and to reduce pro-inflammatory cytokines (IL-6 and TNF-α) in intestinal fluids. In animals instilled with ethanol, mesalazine produced a loss of body weight and intestinal damages with increased IL-6. These side effects were prevented by the co-administration of mesalazine and the LAB mixture. The LAB blend did not affect the primary anti-inflammatory treatment, was able to improve it, and also prevented the side effects of this therapy.


Folate Riboflavin Immune modulation Anti-inflammatory therapy Recurrent colitis 


Funding information

This work was financially supported by CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas projects PIP006 and 0697) and ANPCyT (Agencia Nacional de Promoción Científica y Tecnológica projects 0301, 2554, 2859, and 3045).

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest.

Ethical approval

Animal experiments were approved by the Animal Protection Committee of CERELA and all experiments comply with the current laws of Argentina and international organizations for the use of experimental animals.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Centro de Referencia para Lactobacilos (CERELA-CONICET)TucumánArgentina
  2. 2.Cátedra de Microbiología Superior, Facultad de Bioquímica, Química y FarmaciaUniversidad Nacional de TucumánTucumánArgentina

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