Protective effects of Lactobacillus plantarum against chronic alcohol-induced liver injury in the murine model

  • Tony J. Fang
  • Jiun-Ting Guo
  • Ming-Kuem Lin
  • Meng-Shiou Lee
  • Yen-Lien Chen
  • Wen-Hsin LinEmail author
Applied microbial and cell physiology


Long-term alcohol consumption causes liver injuries such as alcoholic hepatitis, fatty liver, and endotoxemia. Some probiotics were demonstrated to exert beneficial effects in the gastrointestinal tract. The present study was aimed to evaluate the protective effects of Lactobacillus plantarum CMU995 against alcohol-induced liver injury. The mice were orally administered L. plantarum CMU995 for 1 week, followed by the administration of alcohol and different tested substances daily for 6 weeks. The liver injury was examined by measuring the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), malondialdehyde (MDA), anti-oxidative enzyme, endotoxin, inflammatory cytokines, and lipid accumulation in the liver or serum among different groups. L. plantarum CMU995 exhibited beneficial effects on alcohol-induced liver injury via reduction in the serum concentration of AST, ALT, cholesterol, triglycerides, endotoxin, TNF-α, IL-1β, and oxidative stress. Furthermore, we also found that the levels of glutathione (GSH), superoxide dismutase (SOD), and intestinal tight junction protein zonula occludens-1 (ZO-1) were considerably higher in L. plantarum CMU995-fed groups when compared with placebo group. Meanwhile, the protective effects were demonstrated biological gradients as controversial dose-dependent. We speculate that L. plantarum CMU995 inhibited the migration of alcohol-derived endotoxin into the blood and liver, thereby improving the intestinal barrier. The present evidence may provide a novel microbiota-based strategy to prevent the alcohol-induced liver injury.


Lactobacillus plantarum CMU995 Glutathione (GSH) Superoxide dismutase (SOD) Alcohol-induced liver injury 



The authors would like to thank all colleagues and students who contributed to this study. Dr. Tse-Yen, Kenny, and Yang from the Department of Medical Research, China Medical University Hospital, Taichung, Taiwan, supported and performed the necessary revision of English grammar and professional suggestions.

Funding information

This work was funded by grants (NSC 101-2313-B-039-009) from the National Science Council of Taiwan.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

These experiments were performed in accordance with guidelines of the China Medical University Hospital’s Animal Committee, which mentioned that all protocol has been reviewed and approved by the Institutional Animal Care and Use Committee of China Medical University (No.103-216-B).

Supplementary material

253_2019_10122_MOESM1_ESM.pdf (642 kb)
ESM 1 (PDF 641 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Food Science and BiotechnologyNational Chung Hsing UniversityTaichungTaiwan, Republic of China
  2. 2.Department of PharmacyChina Medical UniversityTaichungTaiwan, Republic of China
  3. 3.Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of PharmacyChina Medical UniversityTaichungTaiwan, Republic of China
  4. 4.College of PharmacyChina Medical UniversityTaichungTaiwan, Republic of China

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