Acidic conditions induce the accumulation of orange Monascus pigments during liquid-state fermentation of Monascus ruber M7
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The influence of pH on the biosynthesis of orange Monascus pigments (OMPs) in Monascus ruber M7 was investigated. Under acidic fermentation conditions, pigment mixtures predominantly rich in OMPs were obtained. HPLC analysis revealed the presence of four orange components (O1–O4) and four yellow components (Y1–Y4) in the mixtures, and the dominant ones were O1 and O3, which accounted for 56.0% to 75.9% of the total pigments in the pH range 3–6. Subsequently, O1 and O3 were identified by LC-DAD-ESI/MS as Rubropunctatin and Monascorubrin, respectively. The yield of OMPs was observed to be inversely dependent on pH. At pH 3, large amounts of OMPs with high purity (79.1%) were accumulated. A real-time quantitative PCR analysis revealed that the expression of genes related to the biosynthesis of OMPs in M. ruber M7 was upregulated at acidic pH as compared to neutral pH, and the variation in the level of expression of these genes with pH was consistent with the production of OMPs. These results indicated that the large accumulation of OMPs under acidic condition involved the acidic pH-induced transcription of genes related to the biosynthesis of OMPs. These results would contribute towards the development of an efficient technology for large-scale production of OMPs.
KeywordsMonascus ruber Orange Monascus pigments pH regulation Biosynthesis genes
This work was supported by the Programs of Natural Science Foundation of China (No. 31501453, 31730068 and 31701573), the Program of Science and Technology Department of Hubei Province (No. 2019AHB067), and the Yangtze Fund for Youth Teams of Science and Technology Innovation (No. 2016cqt02).
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Conflict of interest
The authors declare that they have no conflict of interests.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Embaby AM, Hussein MN, Hussein A (2018) Monascus orange and red pigments production by Monascus purpureus ATCC16436 through co-solid state fermentation of corn cob and glycerol: an eco-friendly environmental low cost approach. PLoS One 13(12):e0207755. https://doi.org/10.1371/journal.pone.0207755 CrossRefGoogle Scholar
- Klinsupa W, Phansiri S, Thongpradis P, Yongsmith B, Pothiratana C (2016) Enhancement of yellow pigment production by intraspecific protoplast fusion of Monascus spp. yellow mutant (ade(-)) and white mutant (prototroph). J Biotechnol 217:62–71. https://doi.org/10.1016/j.jbiotec.2015.11.002 CrossRefGoogle Scholar
- Lv J, Zhang BB, Liu XD, Zhang C, Chen L, Xu GR, Cheung PCK (2017) Enhanced production of natural yellow pigments from Monascus purpureus by liquid culture: the relationship between fermentation conditions and mycelial morphology. J Biosci Bioeng 124:452–458. https://doi.org/10.1016/j.jbiosc.2017.05.010 CrossRefGoogle Scholar
- Penalva MA, Arst HNJ (2004) Recent advances in the characterization of ambient pH regulation of gene expression in filamentous fungi and yeasts. Annu Rev Microbiol 58:425–451. https://doi.org/10.1146/annurev.micro.58.030603.123715 CrossRefGoogle Scholar
- Schmitt EK, Kempken R, Kuck U (2001) Functional analysis of promoter sequences of cephalosporin C biosynthesis genes from Acremonium chrysogenum: specific DNA-protein interactions and characterization of the transcription factor PACC. Mol Gen Genomics 265:508–518. https://doi.org/10.1007/s004380000439 CrossRefGoogle Scholar
- Shah AJ, Tilburn J, Adlard MW, Arst HN Jr (1991) pH regulation of penicillin production in Aspergillus nidulans. FEMS Microbiol Lett 61:209–212. https://doi.org/10.1111/j.1574-6968.1991.tb04349.x CrossRefGoogle Scholar
- Suarez T, Penalva MA (1996) Characterization of a Penicillium chrysogenum gene encoding a PacC transcription factor and its binding sites in the divergent pcbAB-pcbC promoter of the penicillin biosynthetic cluster. Mol Microbiol 20:529–540. https://doi.org/10.1046/j.1365-2958.1996.5421065.x CrossRefGoogle Scholar
- Xiong X, Zhang XH, Wu ZQ, Wang ZL (2015a) Accumulation of yellow Monascus pigments by extractive fermentation in nonionic surfactant micelle aqueous solution. Appl Microbiol Biotechnol 99:1173–1180. https://doi.org/10.1007/s00253-014-6227-0
- Xiong X, Zhang XH, Wu ZQ, Wang ZL (2015b) Coupled aminophilic reaction and directed metabolic channeling to red Monascus pigments by extractive fermentation in nonionic surfactant micelle aqueous solution. Process Biochem 50:180–187. https://doi.org/10.1016/j.procbio.2014.12.002 CrossRefGoogle Scholar
- Yongsmith B, Thongpradis P, Klinsupa W, Chantrapornchai W, Haruthaithanasan V (2013) Fermentation and quality of yellow pigments from golden brown rice solid culture by a selected Monascus mutant. Appl Microbiol Biotechnol 97:8895–8902. https://doi.org/10.1007/s00253-013-5106-4 CrossRefGoogle Scholar
- Zhu DQ, Wu JR, Zhan XB, Zhu L, Jiang Y (2019) Enhanced N-acetyl-d-neuraminic production from glycerol and N-acetyl-d-glucosamine by metabolically engineered Escherichia coli with a two-stage pH-shift control strategy. J Ind Microbiol Biotechnol 46:125–132. https://doi.org/10.1007/s10295-018-02132-8 CrossRefGoogle Scholar