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Applied Microbiology and Biotechnology

, Volume 103, Issue 19, pp 7983–7995 | Cite as

Effect of Lactobacillus plantarum expressing multifunctional glycoside hydrolases on the characteristics of alfalfa silage

  • Jingui Guo
  • Yixiao Xie
  • Zhu Yu
  • Geng Meng
  • Zhe WuEmail author
Biotechnological products and process engineering
  • 64 Downloads

Abstract

For the first time, Lactobacillus plantarum strains carrying heterologous genes encoding multifunctional glycoside hydrolases were constructed and used as additives for alfalfa silage. The chemical characteristics, nonstructural carbohydrate composition, and fermentation quality of alfalfa silage were examined. The supernatant of L. plantarum expressing CbXyn10C and Bgxg1 (LP11AG) showed activities on xylan, Avicel, and carboxymethylcellulose (CMC), while the supernatant of the wild-type L. plantarum showed no activity. When LP11AG was used as silage additive, the water-soluble carbohydrate content of alfalfa silage increased by 72%, 55%, and 155% compared with control when the silage was stored at 20 °C, 30 °C, and 40 °C, respectively. With LP11AG being used as an additive for the alfalfa silage stored at 20 °C, the hemicellulose, cellulose, and acid detergent ligninin (ADL) contents decreased by 17%, 6%, and 14% compared with the control (p < 0.05), respectively. Compared with the corresponding original contents, the contents of glucose, arabinose, galactose, and fructose detected in silage treated with LP11AG after 45 days of ensiling increased by 55%, 1494%, 68%, and 5% , respectively, when stored at 40 °C. Raffinose and stachyose, originally present in alfalfa, disappeared after ensiling. In conclusion, our results suggest that LP11AG provides a substantial benefit as a silage additive.

Keywords

Alfalfa silage Lactobacillus plantarum Glycoside hydrolases Nonstructural carbohydrate composition Structural carbohydrate 

Notes

Funding information

This work was supported by the Modern Agro-industry Technology Research System (CARS-34), the National Natural Science Foundation of China (31702181), the National Key Research and Development Program of China (2017YFD0502100), and Fundamental Research Funds for the Central Universities (2018QC048).

Compliance with ethical standards

This article does not contain any studies with human participants or animal experiments.

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary material

253_2019_10097_MOESM1_ESM.pdf (980 kb)
ESM 1 (PDF 979 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Jingui Guo
    • 1
  • Yixiao Xie
    • 1
  • Zhu Yu
    • 1
  • Geng Meng
    • 2
  • Zhe Wu
    • 1
    Email author
  1. 1.College of Grassland Science and TechnologyChina Agricultural UniversityBeijingChina
  2. 2.College of Veterinary MedicineChina Agricultural UniversityBeijingChina

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