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A new β-galactosidase extracted from the infant feces with high hydrolytic and transgalactosylation activity

  • Yongping Xin
  • Tingting Guo
  • Yi Zhang
  • Jiapeng Wu
  • Jian KongEmail author
Biotechnologically relevant enzymes and proteins
  • 48 Downloads

Abstract

A β-galactosidase (β-GalINF) was directly isolated from feces of an 8-month-old infant. Mass spectrum analysis showed β-GalINF with coverage over 50% to the β-galactosidase from Bifidobacterium longum EK3. Accordingly, the β-galINF was amplified from the feces metagenomic DNA by degenerate primers. After overexpressed in Escherichia coli, the β-GalINF was purified and biochemical characterized. β-GalINF existed as homotetramer and homodimer, whose activity (optimal at 50 °C, pH 6.5) was exhilaratingly increased to 484% by artificial intestinal juice. The Km and Vmax values for oNPG and lactose were 20.95 ± 2.76 mM, 5004.50 ± 318.8 μmol min−1 mg−1 and 140.2 ± 17.7 mM, 293.1 ± 14.7 μmol min−1 mg−1, respectively. The production rate of galacto-oligosaccharides by β-GalINF from 20% lactose at 50 °C was 33.4 ± 0.67%. These results suggested the β-GalINF with high hydrolytic and transgalactosylation activity from the infant intestinal has great potential as infant lactase preparation. Moreover, this study provided a new way for exploring undetected enzymes by uncultured-dependent methods.

Keywords

Infant β-Galactosidase Hydrolytic activity Transgalactosylation 

Notes

Funding information

This work was supported by grants from the National Key Research and Development Program of China (2017YFD0400300), the National Natural Science Foundation of China (31871767), and the Public Service Sectors (Agriculture) Special and Scientific Research Projects (201503134).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interests.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all parents of the infants included in the study.

Supplementary material

253_2019_10092_MOESM1_ESM.pdf (156 kb)
ESM 1 (PDF 155 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Microbial TechnologyShandong UniversityQingdaoPeople’s Republic of China
  2. 2.QingdaoPeople’s Republic of China

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