Enhanced calcite precipitation for crack healing by bacteria isolated under low-nitrogen conditions
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A nitrogen-starving isolation strategy was developed for the first time to screen bacteria with high calcium-precipitating activity (CPA) for bioremediation of damage in porous media. Meanwhile, a novel mini-tube method based on the detection of insoluble Ca2+ was established to evaluate the CPA of the isolates. A low-nitrogen-demanding strain B6, identified as Bacillus sp., was screened to exhibit the highest CPA (55 mM insoluble Ca2+). Furthermore, the effects of environmental factors and nutrient availability on B6-induced calcium precipitation were evaluated. The results show that nitrate and starch are the best nitrogen source and carbon source with optimal concentration being 4 and 2 g L−1, respectively. The suitable pH range for B6 to precipitate calcium is from 8.5 to 10.5. B6 can maintain the highest CPA at an initial spore concentration of 1.0 × 108 spores·mL−1. The optimal CaO2 dosage is 10 g L−1. Finally, the calcite precipitation is confirmed by ESEM, EDS, and XRD analysis.
KeywordsLow-nitrogen-demanding bacteria Calcium-precipitating activity Self-healing concrete Bacillus sp. Microbially induced calcium carbonate precipitation
This work was supported by the National Natural Science Foundation of China (No. 51508338, No. 51578339, No. 51120185002) and the Project of Department of Education of Guangdong Province (No. 2017GKTSCX064).
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Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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