Applied Microbiology and Biotechnology

, Volume 103, Issue 17, pp 7191–7202 | Cite as

Improvement of bio-cementation at low temperature based on Bacillus megaterium

  • Xiaohao Sun
  • Linchang MiaoEmail author
  • Linyu Wu
  • Runfa Chen
Environmental biotechnology


A low production rate for calcium carbonate with microbial solidification technology at low temperatures often restricts its application. For this reason, adding urea to the medium and the domestication of Bacillus megaterium at low temperature were proposed to produce more calcium carbonate based on an analysis of growth characteristics, urease activity, and the production rates for calcium carbonate under different conditions. Sand solidification tests were conducted to demonstrate improvements caused by the methods. The results showed that the higher the temperature, the faster the growth of Bacillus megaterium and the stronger the urease activity. Growth was fastest and urease activity strongest at a pH of 8. Adding urea to the medium and the domestication of B. megaterium at low temperature can both improve the production rate, effectively increasing calcium carbonate precipitation at low temperature. Combining the two methods resulted in greater improvement of the production rate for calcium carbonate. The two methods were also found to improve the effect of sand solidification. Therefore, our study provides a solid foundation for the actual engineering application of bio-cementation technology at low temperature.


Bacillus megaterium Low temperature Precipitation rate Bacteria domestication Sand solidification 



The authors thank the valuable comments from the reviewers.


This study was funded by the National Natural Science Foundation of China (grant number 51578147), and Scientific Research Foundation of Graduate School of Southeast University (grant number YBJJ1846). This work was also Supported by “the Fundamental Research Funds for the Central Universities” and “Postgraduate Research & Practice Innovation Program of Jiangsu Province” (grant number KYCX18_0107).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants performed by any of the authors.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Xiaohao Sun
    • 1
  • Linchang Miao
    • 1
    Email author
  • Linyu Wu
    • 1
  • Runfa Chen
    • 1
  1. 1.Institute of Geotechnical EngineeringSoutheast UniversityNanjingChina

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