Bioprospecting microalgae from natural algal bloom for sustainable biomass and biodiesel production
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Natural algal bloom consists of promising algal species which could be a feasible option for the source of bulk biomass and biodiesel production. It has been found in five natural fresh water algal blooms (Uttar Pradesh, India), containing high nitrogen (N) (4.6 ± 0.32 mg/L) and phosphorus (P) (4.12 ± 0.29 mg/L) concentration during spring (23.9–25.9 °C) and summer season (32.0–35.0 °C). Among the isolated algae from naturally occurring bloom, Chlorella sorokiniana MKP01 exhibited highest biomass (1.02 ± 0.02 g/L) and lipid content (174.1 ± 9.6 mg/L) in untreated tap water and urea/single super phosphate (SSP) in the ratio (2:1). The biodiesel quality was assessed and found to be with the Indian and international standards. Algal bloom was artificially developed in the open pond containing 10,000 l tap water supplemented with Urea/SSP (2:1) for a consistent supply of bulk biomass, yielded 8 kg of total biomass and lipid 1.3 kg.
KeywordsAlgal bloom Water parameters Agricultural fertilizer Biomass Biofuels
The authors are thankful to the Director, CSIR-National Botanical Research Institute, Lucknow for the constant encouragement and necessary laboratory facilities. The authors acknowledge all lab members for their support.
The Department of Biotechnology (DBT), Delhi, India provided financial support.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any experiments involving human participants or animals performed by any of the authors.
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