The effect of reactive oxygen species (ROS) and ROS-scavenging enzymes, superoxide dismutase and catalase, on the thermotolerant ability of Corynebacterium glutamicum
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The function of two reactive oxygen species (ROS) scavenging enzymes, superoxide dismutase (SOD) and catalase, on the thermotolerant ability of Corynebacterium glutamicum was investigated. In this study, the elevation of the growth temperature was shown to lead an increased intracellular ROS for two strains of Corynebacterium glutamicum, the wild-type (KY9002) and the temperature-sensitive mutant (KY9714). In order to examine the effects of ROS-scavenging enzymes on cell growth, either the SOD or the catalase gene was disrupted or overexpressed in KY9002 and KY9714. In the case of the KY9714 strain, it was shown that the disruption of SOD and catalase disturbs cell growth, while the over-productions of both the enzymes enhances cell growth with a growth temperature of 30 °C and 33 °C. Whereas, in the relatively thermotolerant KY9002 strain, the disruption of both enzymes exhibited growth defects more intensively at higher growth temperatures (37 °C or 39 °C), while the overexpression of at least SOD enhanced the cell growth at higher temperatures. Based on the correlation between the cell growth and ROS level, it was suggested that impairment of cell growth in SOD or catalase-disrupted strains could be a result of an increased ROS level. In contrast, the improvement in cell growth for strains with overexpressed SOD or catalase resulted from a decrease in the ROS level, especially at higher growth temperatures. Thus, SOD and catalase might play a crucial role in the thermotolerant ability of C. glutamicum by reducing ROS-induced temperature stress from higher growth temperatures.
KeywordsCorynebacterium glutamicum Reactive oxygen species (ROS) Superoxide dismutase (SOD) Catalase Thermotolerant
The authors wish to thank Sayoko Tanigawa and Seisuke Hatakeyama for their technical support. Part of this work was performed through collaboration with the Core to Core Program, which was supported by the Japan Society for the Promotion of Science (JSPS) and the National Research Council of Thailand (NRCT).
This work was supported financially by the Advanced Low Carbon Technology Research and Development Program (ALCA: JPMJAL1106) of Japan Science and Technology Agency (JST).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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