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Applied Microbiology and Biotechnology

, Volume 103, Issue 13, pp 5379–5390 | Cite as

Trehalose induced by reactive oxygen species relieved the radial growth defects of Pleurotus ostreatus under heat stress

  • Min Lei
  • Xiangli Wu
  • Chenyang Huang
  • Zhiheng Qiu
  • Lining Wang
  • Ruiying Zhang
  • Jinxia ZhangEmail author
Applied microbial and cell physiology
  • 131 Downloads

Abstract

Trehalose is a nonreducing disaccharide, and it plays an intracellular protective role in organisms under various stress conditions. In this study, the trehalose synthesis and its protective role in Pleurotus ostreatus were investigated. As a signal in metabolic regulation, reactive oxygen species (ROS) accumulated in the mycelia of P. ostreatus under heat stress (HS). Furthermore, mycelial growth was significantly inhibited, and the malondialdehyde (MDA) level significantly increased under HS. First, exogenous addition of H2O2 inhibited mycelial growth and elevated the MDA level, while N-acetyl cysteine (NAC) and vitamin C (VC) reduced the MDA level and recovered mycelial growth under HS by scavenging ROS. These results indicated that the mycelial radial growth defect under HS might be partly caused by ROS accumulation. Second, adding NAC and VC to the media resulted in rescued trehalose accumulation, which indicated that ROS has an effect on inducing trehalose synthesis. Third, the mycelial growth was recovered by addition of trehalose to the media after HS, and the MDA level was reduced. This effect was further verified by the overexpression of genes for trehalose-6-phosphate synthase (TPS) and neutral trehalase (NTH), which led to increased and reduced trehalose content, respectively. In addition, adding validamycin A (NTH inhibitor) to the media promoted trehalose accumulation and the recovered mycelial growth after HS. In conclusion, trehalose production was partly induced by ROS accumulation in the mycelia under HS, and the accumulated trehalose could promote the recovery of growth after HS, partly by reducing the MDA level in the mycelia.

Keywords

Trehalose Heat stress Growth inhibition rate MDA ROS 

Notes

Acknowledgments

Special thanks are given to Prof. Mingwen Zhao (Nanjing Agricultural University, China) for his excellent technical instruction and critical comments on this manuscript.

Funding information

This research was funded by the National Basic Research Program of China (2014CB138303), the China Agriculture Research System (CARS20), the National Natural Science Foundation of China (31601803), and the Fundamental Research Funds for Central Nonprofit Scientific Institution (1610132016048).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

253_2019_9834_MOESM1_ESM.pdf (855 kb)
ESM 1 (PDF 855 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Min Lei
    • 1
  • Xiangli Wu
    • 1
  • Chenyang Huang
    • 1
  • Zhiheng Qiu
    • 1
  • Lining Wang
    • 1
  • Ruiying Zhang
    • 1
  • Jinxia Zhang
    • 1
    Email author
  1. 1.Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences; Key Laboratory of Microbial ResourcesMinistry of Agriculture and Rural AffairsBeijingChina

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