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Regulation of teicoplanin biosynthesis: refining the roles of tei cluster-situated regulatory genes

  • Oleksandr Yushchuk
  • Liliya Horbal
  • Bohdan Ostash
  • Flavia Marinelli
  • Wolfgang Wohlleben
  • Evi Stegmann
  • Victor FedorenkoEmail author
Applied genetics and molecular biotechnology
  • 113 Downloads

Abstract

Teicoplanin is a frontline glycopeptide antibiotic produced by Actinoplanes teichomyceticus. It is used to treat complicated cases of infection, including pediatric ones, caused by Gram-positive pathogens. There is a steady interest in elucidating the genetic mechanisms determining teicoplanin production, as they would help overproduce known teicoplanins and discover novel glycopeptides. Herein, we investigate the transcriptional organization of the tei biosynthetic gene cluster and the roles of the cluster-situated regulatory genes in controlling teicoplanin production and self-resistance in A. teichomyceticus. We demonstrate that the tei cluster is organized into nine polygenic and nine monogenic transcriptional units. Most of tei biosynthetic genes are subjected to StrR-like Tei15* control, which, in turn, appears to be regulated by LuxR-type Tei16*. Expression of the genes conferring teicoplanin self-resistance in A. teichomyceticus is not co-regulated with antibiotic production. The gene tei31*, coding for a putative DNA binding protein, is not expressed under teicoplanin producing conditions and is dispensable for antibiotic production. Finally, phylogenesis reconstruction of the glycopeptide cluster-encoded regulators reveals two main clades of StrR-like regulators. Tei15* and close orthologues form one of these clades; the second clade is composed by orthologues of Bbr and Dbv4, governing the biosynthesis of balhimycin and teicoplanin-like A40926, respectively. In addition, the LuxR-type Tei16* appears unrelated to the LuxR-like Dbv3, which is controlling A40926 biosynthesis. Our results shed new light on teicoplanin biosynthesis regulation and on the evolution of novel and old glycopeptide biosynthetic gene clusters.

Keywords

Actinoplanes Glycopeptide antibiotics Glycopeptide resistance Teicoplanin Cluster-situated regulatory genes tei cluster 

Notes

Funding

This work was supported by grant Bg-46F from the Ministry of Education and Science of Ukraine to V. F., personal grants from CIB (Consorzio Interuniversitario per le Biotecnologie) and DAAD (German Academic Exchange Service) 57048249 to O.Y., and support from the DFG (SFB 766) to E.S.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical statement

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

253_2019_9789_MOESM1_ESM.pdf (713 kb)
ESM 1 (PDF 713 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Genetics and BiotechnologyIvan Franko National University of LvivLvivUkraine
  2. 2.Department of Pharmaceutical Biotechnology, Helmholtz Institute for Pharmaceutical Research Saarland, Actinobacteria Metabolic Engineering GroupSaarland UniversitySaarbruckenGermany
  3. 3.Department of Biotechnology and Life SciencesUniversity of InsubriaVareseItaly
  4. 4.Interfaculty Institute of Microbiology and Infection Medicine Tübingen, Microbiology/BiotechnologyUniversity of TübingenTubingenGermany

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