Advertisement

Beer yeast-derived fluorescent carbon dots for photoinduced bactericidal functions and multicolor imaging of bacteria

  • Zhe Gao
  • Chun-xi Zhao
  • Yan-yan Li
  • Ya-ling YangEmail author
Environmental biotechnology
  • 60 Downloads

Abstract

Beer yeast-modified fluorescent carbon dots were synthesized via a one-step strategy for photoinduced bactericidal functions and bio-imaging in bacterial viability assessment. The proposed carbon dots (CDs) were used as an visible light-triggered antibacterial material, and the antimicrobial activities of the CDs against Gram-negative model bacterial species (Escherichia coli) were evaluated under conditions of varying other experimental parameters including CDs concentrations and treatment times. The result showed that the CDs have excellent antibacterial performance of bactericidal effect within 120 min of under visible-light irradiation. And the bactericidal efficiency increased with the increasing concentration of CDs and visible-light illumination time. Moreover, the CDs with high quantum yield (21%) possess highly negative zeta potential (− 41.7 mV) and low cytotoxicity, the CDs could serve as an efficient dye for bacterial viability evaluation, they could selectively stain dead E. coli rather than live ones, which make dead E. coli be viewed with multicolor fluorescence under different excitation wavelengths.

Keywords

Carbon dots Antibacterial Bio-imaging Escherichia coli Multicolor fluorescence 

Notes

Funding information

The work was strongly supported by the Analysis and Testing Foundation of Kunming University of Science and Technology (2018M20172118081).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

253_2019_9782_MOESM1_ESM.pdf (304 kb)
ESM 1 (PDF 304 kb)

References

  1. Atchudan R, Tnji E, Aseer KR, Perumal S, Karthik N, Lee YR (2017) Highly fluorescent nitrogen-doped carbon dots derived from Phyllanthus acidus utilized as a fluorescent probe for label-free selective detection of Fe3+ ions, live cell imaging and fluorescent ink. Biosens Bioelectron 99:303–311CrossRefGoogle Scholar
  2. Borse V, Thakur M, Sengupta S, Srivastava R (2017) N-doped multi-fluorescent carbon dots for ‘turn off-on’ silver-biothiol dual sensing and mammalian cell imaging application. Sensors Actuators B Chem 248:481–492CrossRefGoogle Scholar
  3. Carvalho F, George J, Sheikh H, Selvin R (2018) Advances in screening, detection and enumeration of Escherichia coli using nanotechnology-based methods: a review. J Biomed Nanotechnol 14:829–846CrossRefGoogle Scholar
  4. Chen JC, Liu JH, Li JZ, Xu LQ, Qiao YJ (2017) One-pot synthesis of nitrogen and sulfur co-doped carbon dots and its application for sensor and multicolor cellular imaging. J Colloid Interface Sci 485:167–174CrossRefGoogle Scholar
  5. Choi Y, Kim S, Choi MH, Ryoo SR, Park J, Min DH, Kim BS (2015) Highly biocompatible carbon Nanodots for simultaneous bioimaging and targeted photodynamic therapy in vitro and in vivo. Adv Funct Mater 24:5781–5789CrossRefGoogle Scholar
  6. Das P, Ganguly S, Bose M, Mondal S, Choudhary S, Gangopadhyay S, Das AK, Banerjee S, Das NC (2018) Zinc and nitrogen ornamented bluish white luminescent carbon dots for engrossing bacteriostatic activity and Fenton based bio-sensor. Mater Sci Eng C 88:115–129CrossRefGoogle Scholar
  7. Ding H, Cheng LW, Ma YY, Kong JL, Xiong HM (2013) Luminescent carbon quantum dots and their application in cell imaging. New J Chem 37:2515–2520CrossRefGoogle Scholar
  8. Fernando KA, Sahu S, Liu Y, Lewis WK, Guliants EA, Jafariyan A, Wang P, Bunker CE, Sun YP (2015) Carbon quantum dots and applications in photocatalytic energy conversion. ACS Appl Mater Interfaces 7:8363–8376CrossRefGoogle Scholar
  9. Gan Z, Xu H, Hao Y (2016) Mechanism for excitation-dependent photoluminescence from graphene quantum dots and other graphene oxide derivates: consensus, debates and challenges. Nanoscale 8:7794–7807CrossRefGoogle Scholar
  10. Gao XH, Lu YZ, Zhang RZ, He SJ, Ju J, Liu MM, Li L, Chen W (2015) One-pot synthesis of carbon nanodots for fluorescence turn-on detection of Ag+ based on the Ag+-induced enhancement of fluorescence. J Mater Chem C 3:2302–2309CrossRefGoogle Scholar
  11. Gu D, Shang SM, Yu Q, Jie S (2016) Green synthesis of nitrogen-doped carbon dots from lotus root for Hg(II) ions detection and cell imaging. Appl Surf Sci 390:38–42CrossRefGoogle Scholar
  12. Hassan MS, Khan R, Amna T, Yang J, Lee IH, Sun MY, El-Newehy MH, Al-Deyab SS, Khil MS (2016) The influence of synthesis method on size and toxicity of CeO2 quantum dots: potential in the environmental remediation. Ceram Int 42:576–582CrossRefGoogle Scholar
  13. Jhonsi MA, Ananth DA, Nambirajan G, Sivasudha T, Yamini R, Bera S, Kathiravan A (2018) Antimicrobial activity, cytotoxicity and DNA binding studies of carbon dots. Spectrochim Acta A Mol Biomol Spectrosc 196:295–302CrossRefGoogle Scholar
  14. King T, Osmond-Mcleod MJ, Duffy LL (2017) Nanotechnology in the food sector and potential applications for the poultry industry. Trends Food Sci Technol 72:62–73CrossRefGoogle Scholar
  15. Kiwi J, Nadtochenko V (2005) Evidence for the mechanism of photocatalytic degradation of the bacterial wall membrane at the TiO2 interface by ATR-FTIR and laser kinetic spectroscopy. Langmuir 21:4631–4641CrossRefGoogle Scholar
  16. Krysmann MJ, Kelarakis A, Dallas P, Giannelis EP (2011) Formation mechanism of carbogenic nanoparticles with dual photoluminescence emission. J Am Chem Soc 134:747–750CrossRefGoogle Scholar
  17. Lu F, Song YX, Huang H, Liu Y, Fu YJ, Huang J, Li H, Qu HH, Kang ZH (2017) Fluorescent carbon dots with tunable negative charges for bio-imaging in bacterial viability assessment. Carbon 120:95–102CrossRefGoogle Scholar
  18. Mehta VN, Jha S, Kailasa SK (2014) One-pot green synthesis of carbon dots by using Saccharum officinarum juice for fluorescent imaging of bacteria (Escherichia coli) and yeast (Saccharomyces cerevisiae) cells. Mater Sci Eng C 38:20–27CrossRefGoogle Scholar
  19. Okkyoung C, Zhiqiang H (2008) Size dependent and reactive oxygen species related nanosilver toxicity to nitrifying bacteria. Environ Sci Technol 42:4583–4588CrossRefGoogle Scholar
  20. Pal T, Mohiyuddin S, Packirisamy G (2018) Facile and green synthesis of multicolor fluorescence carbon dots from curcumin: In Vitro and in Vivo bioimaging and other applications. ACS Omega 3:831–843CrossRefGoogle Scholar
  21. Ren G, Zhang Q, Li S, Fu SS, Chai F, Wang CG, Qu FY (2017) One pot synthesis of highly fluorescent N doped C-dots and used as fluorescent probe detection for Hg2+ and Ag+ in aqueous solution. Sensors Actuators B Chem 243:244–253CrossRefGoogle Scholar
  22. Renuka RM, Achuth J, Chandan HR, Venkataramana M, Kadirvelu K (2018) A fluorescent dual aptasensor for the rapid and sensitive onsite detection of E. coli O157:H7 and its validation in various food matrices. New J Chem 42:10807–10817CrossRefGoogle Scholar
  23. Safardoust-Hojaghan H, Salavati-Niasari M, Amiri O, Hassanpour M (2017) Preparation of highly luminescent nitrogen doped graphene quantum dots and their application as a probe for detection of Staphylococcus aureus and E. coli. J Mol Liq 241:1114–1119CrossRefGoogle Scholar
  24. Srivastava AK, Dev A, Karmakar S (2017) Nanosensors and nanobiosensors in food and agriculture. Environ Chem Lett 16:161–182CrossRefGoogle Scholar
  25. Viana OS, Ribeiro MS, Rodas ACD, Rebouças JS, Adriana F, Santos BS (2015) Comparative study on the efficiency of the photodynamic inactivation of Candida albicans using CdTe quantum dots, Zn(II) porphyrin and their conjugates as photosensitizers. Molecules 20:8893–8912CrossRefGoogle Scholar
  26. Wang HT, Liu S, Xie YS, Bi JR, Li Y, Song YK, Cheng SS, Li DM, Tan MQ (2018) Facile one-step synthesis of highly luminescent N-doped carbon dots as efficient fluorescence probe for chromium (VI) detection based on inner filter effect. New J Chem 42:3729–3735CrossRefGoogle Scholar
  27. Wu P, Huang RX, Li GY, He YY, Chen CM, Xiao W, Ding P (2018) Optimization of synthesis and modification of ZnSe/ZnS quantum dots for fluorescence detection of Escherichia coil. J Nanosci Nanotechnol 18:3654–3659CrossRefGoogle Scholar
  28. Xu JW, Gao ZD, Han K, Liu YM, Song YY (2014) Synthesis of magnetically separable Ag3PO4/TiO2/Fe3O4 heterostructure with enhanced photocatalytic performance under visible light for photoinactivation of bacteria. ACS Appl Mater Interfaces 6:15122–15131CrossRefGoogle Scholar
  29. Xu YG, Liu QQ, Liu CC, Zhai YP, Xie M, Huang LY, Xu H, Li HM, Jing JJ (2018) Visible-light-driven ag/AgBr/ZnFe2O4 composites with excellent photocatalytic activity for E. coli disinfection and organic pollutant degradation. J Colloid Interface Sci 512:555–566CrossRefGoogle Scholar
  30. Xue L, Zheng LY, Zhang HL, Jin X, Lin JH (2018) An ultrasensitive fluorescent biosensor using high gradient magnetic separation and quantum dots for fast detection of foodborne pathogenic bacteria. Sensors Actuators B Chem 265:318–325CrossRefGoogle Scholar
  31. Yan FY, Shi DC, Zheng TC, Yun KY, Zhou XG, Chen L (2016) Carbon dots as nanosensor for sensitive and selective detection of Hg2+ and L-cysteine by means of fluorescence “off–on” switching. Sensors Actuators B Chem 224:926–935CrossRefGoogle Scholar
  32. Yang JJ, Zhang XD, Ma YH, Gao G, Chen XK, Jia HR, Li YH, Chen Z, Wu FG (2016) Carbon dot-based platform for simultaneous bacterial Distinguishment and antibacterial applications. ACS Appl Mater Interfaces 8:32170–32181CrossRefGoogle Scholar
  33. Zhang YY, Wang LL, Yang ML, Wang J, Shi JS (2019) Carbon quantum dots sensitized ZnSn(OH)6 for visible light-driven photocatalytic water purification. Appl Surf Sci 466:515–524Google Scholar
  34. Zhou Z, Wang FY, Yang GC, Lu CF, Nie JQ, Chen ZX, Ren J, Sun Q, Zhao CC, Zhu WH (2017) A Ratiometric fluorescent probe for monitoring leucine aminopeptidase in living cells and zebrafish model. Anal Chem 89:11576–11582CrossRefGoogle Scholar
  35. Zhu JH, Li MM, Liu SP, Liu ZF, Li YF, Hu XL (2015) Fluorescent carbon dots for auramine O determination and logic gate operation. Sensors Actuators B Chem 219:261–267CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Faculty of Life Science and TechnologyKunming University of Science and TechnologyKunmingChina

Personalised recommendations