Applied Microbiology and Biotechnology

, Volume 103, Issue 8, pp 3317–3326 | Cite as

Attributes of Polygonum multiflorum to transfigure red biotechnology

  • Thanh-Tam Ho
  • Hosakatte Niranjana MurthyEmail author
  • Dayanand Dalawai
  • Medha A. Bhat
  • Kee-Yoeup Paek
  • So-Young ParkEmail author


A vast array of plant-based compounds has enriched red biotechnology to serve the human health and food. A peculiar medicinal plant which was an element of traditional Chinese medicine for centuries as a liver and kidney tonic, for life longevity and hair blackening, is Polygonum multiflorum Thunb. (PM) which is popularly known as “He shou wu” or “Fo-ti” and is rich in chemical components like stilbenes, quinones, and flavonoids which have been used as anti-aging, anti-alopecia, anti-cancer, anti-oxidative, anti-bacterial, anti-hyperlipidemia, anti-atherosclerosis, and immunomodulating and hepatoprotective agents in the modern medicine. The health benefits from PM are attained since long through commercial products such as PM root powder, extract, capsules, tincture, shampoo, and body sprays in the market. Currently, the production of these pharmaceuticals and functional foods possessing stilbenes, quinones, and flavonoids is through cell and organ cultures to meet the commercial demand. However, hepatotoxic effects of PM-based products are the stumbling blocks for its long-term usage. The current review encompasses a comprehensive account of bioactive compounds of PM roots, their biological activities as well as efficacy and toxicity issues of PM ingredients and future perspectives.


Fo-ti He shou wu Polygonum multiflorum Red biotechnology 


Funding information

This study was funded by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through Advanced Production Technology Development Program, funded by the Ministry of Agriculture, Food and Rural Affairs (Grant Number 315013-4).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.


  1. Anonymous (2018) USDA, Agricultural Research Service, National Plant Germplasm System. 2018. Germplasm resources information network (GRIN-taxonomy). National Germplasm Resources Laboratory, Beltsville, Maryland. URL:
  2. Bounda GA, Feng YU (2015) Review of clinical studies of Polygonum multiflorum Thunb. and its isolated bioactive compounds. Pharm Res 7:225–236Google Scholar
  3. Caro Y, Anamale L, Fouillaud M, Laurent P, Petit T, Dufosse L (2012) Natural hydroxyanthraquinonoid pigments as potent food grade colorants: an overview. Nat Prod Bioprospect 2:174–193CrossRefGoogle Scholar
  4. Cha DS, Jeon H (2009) Anti-inflammatory effect of MeOH extracts of the stem of Polygonum multiflorum in LPS-stimulated mouse peritoneal macrophages. Nat Prod Sci 15:83–89Google Scholar
  5. Chan YC, Wang MF, Chen YC, Yang DY, Lee MS, Cheng FC (2003) Long-term administration of Polygonum multiflorum Thunb. reduces cerebral ischemia induced infarct volume in gerbils. Am J Chin Med 31:71–77CrossRefGoogle Scholar
  6. Chen Z (2015) Heshouwu. In: Chen Z (ed) Commission of Chinese Pharmacopeia 2015. Pharmacopeia of People’s republic of China. 1. China medical. Science Press, Beijing, pp 175–177Google Scholar
  7. Chen QT, Zhou LH, Xu W, Huang ZH, Qiu XH (2012) Content changes of 5 components in Polygonum multiflorum during processing. Chin J Exp Tradit Med Form 18:66–71Google Scholar
  8. Dong X, Fu J, Yin X, Cao S, Li X, Lin L, Huvilgegi NJ (2016) Emodin: a review of its pharmacology, toxicity and pharmacokinetics. Phytother Res 30:1207–1218CrossRefGoogle Scholar
  9. Han YS, Van der Heijden R, Verpoorte R (2001) Biosynthesis of anthraquinones in cell cultures of the Rubiaceae. Plant Cell Tissue Organ Cult 67:201–220CrossRefGoogle Scholar
  10. Ho TT, Lee KJ, Lee JD, Bhushan S, Paek KY, Park SY (2017) Adventitious root culture of Polygonum multiflorum for phenolic compounds and its pilot-scale production in 500 L-tank. Plant Cell Tissue Organ Cult 130:167–181CrossRefGoogle Scholar
  11. Ho TT, Lee JD, Ahn MS, Kim SW, Park SW (2018a) Enhanced production of phenolic compounds in hairy root cultures of Polygonum multiflorum and its metabolite discrimination using HPLC and FT-IR methods. Appl Microbiol Biotechnol 102:9563–9575CrossRefGoogle Scholar
  12. Ho TT, Lee JD, Jeong CS, Paek KY, Park SY (2018b) Improvement of biosynthesis and accumulation of bioactive compounds by elicitation in adventitious root cultures of Polygonum multiflorum. Appl Microbiol Biotechnol 102:199–209CrossRefGoogle Scholar
  13. Hsu SH, Chung JG (2012) Anticancer potential of emodin. BioMedicine 2:108–116CrossRefGoogle Scholar
  14. Huang CH, Horng LY, Chen CF, Wu RT (2007) Chinese herb Radix Polygoni Multiflori as a therapeutic drug for liver cirrhosis in mice. J Ethanopharmacol 114:199–206CrossRefGoogle Scholar
  15. Huang B, Lin H, Yan C, Qiu H, Qiu L, Yu R (2014) Optimal inductive and cultural conditions of Polygonum multiflorum transgenic hairy roots mediated with Agrobacterium rhizogenes R1601 and analysis of their anthraquinone constituents. Phrmacogn Mag 10:77-82Google Scholar
  16. Ip SP, Tse A, Poon MK, Kow KM, Ma CY (1997) Antioxidant activities of Polygonum multiflorum Thunb. in vivo and in vitro. Phytother Res 11:42–44CrossRefGoogle Scholar
  17. Lee BH, Huang YY, Duh PD, Wu SC (2012) Hepatoprotection of emodin and Polygonum multiflorum against CCl(4)-induced liver injury. Pharm Biol 50:351–359CrossRefGoogle Scholar
  18. Li C, Cai F, Yang Y, Zhao X, Wang C, Li J, Jia Y, Tang J, Liu Q (2010) Tetrahydroxystilbene glucoside ameliorates diabetic nephrophathy in rats: involvement of SIRT1 and TGF-β1 pathway. Eur J Pharmacol 459:382–389CrossRefGoogle Scholar
  19. Li H, Wang X, Liu Y, Pan D, Wang Y, Yang N, Xiang L, Cai X, Feng Y (2017) Hepatoprotection and hepatotoxicity of Heshouwu, a Chinese medicinal herb: context of the paradoxial effect. Food Chem Toxicol 108:407–418CrossRefGoogle Scholar
  20. Lin LC, Nalawade SM, Mulabagal V, Yeh MS, Tsay HS (2003) Micropropagation of Polygonum multiflorum Thunb and quantitative analysis of the anthraquinones emodin and physcion formed in in vitro propagated shoots and plants. Biol Pharm Bull 26:1467–1471CrossRefGoogle Scholar
  21. Lin HW, Sum MX, Wang YH, Yang LM, Yan YR, Huang N, Xuan LJ, Xu YM, Bai DL, Zheng YT, Xiao K (2010) Anti-HIV activities of the compounds isolated from Polygonum cuspidatum and Polygonum multiflorum. Planta Med 76:889–892CrossRefGoogle Scholar
  22. Lin L, Ni B, Lin H, Zhang M, Li X, Yin X, Qu C, Ni J (2015) Traditional usages, botany, phytochemistry, pharmacology and toxicology of Polygonum multiflorum Thunb.: a review. J Ethnopharmacol 159:158–183CrossRefGoogle Scholar
  23. Ling S, Xu JW (2016) Biological activities of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside in antiaging and antiaging-related disease treatments. Oxidative Med Cell Longev 2016:1–4Google Scholar
  24. Liu QL, Xao JH, Ma R, Ban Y, Wang JL (2007) Effect of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside on lipoprotein oxidation and proliferation of coronary arterial smooth cells. J Asian Nat Prod Res 9:689–697CrossRefGoogle Scholar
  25. Liu Z, Chao Z, Liu Y, Song Z, Lu A (2009) Maillard reaction involved in the steaming process of the root of Polygonum multiflorum. Planta Med 75:84–88CrossRefGoogle Scholar
  26. Liu Y, Wang Q, Yang J, Guo X, Liu W, Ma S, Li S (2018) Polygonum multiflorum Thunb.: a review on chemical analysis, processing mechanism, quality evaluation, and hepatotoxicity. Front Pharmacol 9:1–16CrossRefGoogle Scholar
  27. Luo YY, Liu JX, Liu XH, Lan CW, Hou Y, Ma Y, Wang SN (2014) Determination of the dynamic changes of in-organic elements in polygoni multifolori radix by ICP-MS. Chin Pharm J 49:1978–1182Google Scholar
  28. Lv LS, Gu XH, Tang J, Ho CT (2007) Antioxidant activity of stilbene glycoside from Polygonum multiflorum Thunb. in vivo. Food Chem 114:1678–1681CrossRefGoogle Scholar
  29. Lv GP, Meng LZ, Han DQ, LI HY, Zhao J, Li SP (2015) Effect of sample preparation on components and liver toxicity of Polygonum multiflorum. J Pharma Biomed Anal 109:105–111CrossRefGoogle Scholar
  30. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–479CrossRefGoogle Scholar
  31. Murthy HN, Hahn EJ, Paek KY (2008) Adventitious roots and secondary metabolism. Chin J Biotechnol 24:711–716CrossRefGoogle Scholar
  32. Murthy HN, Lee EJ, Paek KY (2014) Production of secondary metabolites from cell and organ cultures: strategies and approaches for biomass improvement and metabolite accumulation. Plant Cell Tissue Organ Cult 118:1–16CrossRefGoogle Scholar
  33. Murthy HN, Dandin VS, Paek KY (2016) Tools for biotechnological production of useful phytochemicals from adventitious root cultures. Phytochem Rev 15:129–145CrossRefGoogle Scholar
  34. Navarro G, Martiniez-Pinila E, Ortiz R, No’e V, Ciudad CJ, Franco R (2018) Resveratrol and related stilbenoids nutraceutical/dietary complements with health-promoting actions: industrial production, safety, and the search for mode of action. Compr Rev Food Sci Food Saf 17:808–826CrossRefGoogle Scholar
  35. Richard T, Pawlus AD, Igl’esias ML, Pedrot E, Waffo-Teguo P, Merillon JM, Monti JP (2011) Neuroprotective properties of resveratrol and derivatives. Ann N Y Acad Sci 1215:103–108CrossRefGoogle Scholar
  36. Seo SR, Kang G, Ha GW, Kim JC (2013) In vivo hair growth-promoting efficacies of herbal extracts and their cubsomal suspensions. J Ind Eng Chem 19:1331–1139CrossRefGoogle Scholar
  37. Shao L, Zhao SJ, Cui TB, Liu ZY, Zhao W (2012) 2, 3, 5, 4′- Tetrahydroxystilbene-2-O-β-D glycoside biosynthesis by suspension cells cultures of Polygonum multiflorum Thunb. and production enhancement by methyl jasmonate and salicylic acid. Molecules 17:2240–2247CrossRefGoogle Scholar
  38. Shi K, Xie J, Luo LJ, Yang HL, Zhang Y (2011) ASS determination of iron black in radix Polygoni multiflori preparata. Chin J Pharm Anal 31:583–585Google Scholar
  39. Song HB, Du XX, Guo XX, Ren JT, Yang L, Pang Y (2015) Safety and risk factor analysis of Polygonum mutiflori Radix base on ancient traditional Chinese medicine literatures. Chin J Chin Mater Med 40:985–988Google Scholar
  40. Thiruvengadam M, Nagella P, Kim EH, Kim SH, Chung IM (2014) Production of anthraquinones, phenolic compounds and biological activities from hairy root cutlurs of Polygonum multiflorum Thunb. Protoplasma 251:555–566CrossRefGoogle Scholar
  41. Thiruvengadam M, Rekha K, Rajakumar G, Lee TJ, Kim SH, Chung IM (2016) Enhanced production of anthraquinones and phenolic compounds and biological activities in the cell suspension cultures of Polygonum multiflorum. Int J Mol Sci 17:1–16CrossRefGoogle Scholar
  42. Verpoorte R, Contin A, Memelink J (2002) Biotechnology for the production of plant secondary metabolites. Phytochem Rev 1:13–25CrossRefGoogle Scholar
  43. Wang R, Tang Y, Feng B, Ye C, Feng L, Zhang L, Li L (2007) Changes in hippocampal synapses and learning-memory abilities in age-increasing rats and effects of tetrahyroxystilbene glucoside in aged rats. Neuroscince 149:739–746CrossRefGoogle Scholar
  44. Wang H, Song L, Feng S, Liu Y, Zuo G, Lai F, He G, Chen M, Huang D (2013) Characterization of proanthocyanidins in stems of Polygonum multiflorum Thunb. as strong starch hydrolase inhibitors. Molecules 18:2255–2265CrossRefGoogle Scholar
  45. Wu X, Chen X, Huang Q, Fang D, Li G, Zhang G (2012) Toxicity of raw and processed roots of Polygonum multiflroum. Fitroterapia 83:469–475CrossRefGoogle Scholar
  46. Xie W, Zhao Y, Du L (2012) Emerging approaches of traditional Chinese medicine formulas for the treatment of hyperlipidemia. J Ethnopharmacol 140:345–367CrossRefGoogle Scholar
  47. Yang LJ (2008) Inssussion on application of hair-blacking and hair growth accelerating effects of Polygonum multiflorum from ancient prescription. J Tradit Chin Med 7:39–40Google Scholar
  48. Yang PY, Almofti MR, Lu L, Kang H, Zhang J, Li TJ, Rui YC, Sun LN, Chen WS (2005) Reduction of atherosclerosis in cholesterol-fed rabbits and decrease of expression of intracellular adhesion molecule-1 and vascular endothelial growth factor in foam cells by water soluble fraction of Polygonum multiflorum. J Pharmacol Sci 99:294–300CrossRefGoogle Scholar
  49. Yu GQ (2014) The processing history of Polygonum multiflorum. J North Pharm 11:63–64Google Scholar
  50. Yu RM, Ma N, Yan CY, Zhao Y (2006) Effects of exogenous phytohormones on hairy root growth of Polygonum multiflorum and biosynthesis of anthraquinones in its hairy root cultures. Chin J Biotechnol 22:619–623CrossRefGoogle Scholar
  51. Yu J, Xie J, Mao XJ, Wang MJ, Li N, Wang J, Zhaori GT, Zhao RH (2011) Hepatotoxicity of major constituents and extractions of Radix Polygoni Multiflori and Radix Polygoni Multiflori Praeparata. J Ethnopharmacol 137:1291–1299CrossRefGoogle Scholar
  52. Zhang L, Xing Y, Ye CF, Ai HX, Wei HF, Li L (2006) Learning memory deficit with aging in APP transgenic mice of Alzheimer’s disease and intervention by using tetrhydroxystilbene glucoside. Behav Brain Res 173:246–254CrossRefGoogle Scholar
  53. Zhang YZ, Shen JF, Xu JY, Xiao JY, Wang JL (2007) Inhibitory effects of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside on experimental inflammation and cyclooxygenase 2 activity. J Asian Nat Prod Res 9:355–363CrossRefGoogle Scholar
  54. Zhou Y, Luo CJ, Deng ZJ (2010) The processing history research of Radix Polygoni Multiflori. China Medical Herald 7:9–10Google Scholar
  55. Zhou YX, Xia W, Yue W, Peng C, Rahman K, Zhang H (2015) Rhein: a review of pharmacological activities Evid Based Complement Alternat Med 578107Google Scholar
  56. Zuo GY, Wang GC, Zhao YB, Xu GL, Hao XY, Han J, Zhao Q (2008) Screening of Chinese medicinal plant for inhibition against clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA). J Ethnopharmacol 120:267–290CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Thanh-Tam Ho
    • 1
  • Hosakatte Niranjana Murthy
    • 2
    Email author
  • Dayanand Dalawai
    • 2
  • Medha A. Bhat
    • 2
  • Kee-Yoeup Paek
    • 1
  • So-Young Park
    • 1
    Email author
  1. 1.Department of Horticulture, Division of Animal, Horticultural and Food SciencesChungbuk National UniversityChenogjuRepublic of Korea
  2. 2.Department of BotanyKarnatak UniversityDharwadIndia

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