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Applied Microbiology and Biotechnology

, Volume 103, Issue 8, pp 3421–3437 | Cite as

Biochemical profiles of two thermostable and organic solvent–tolerant esterases derived from a compost metagenome

  • Mingji Lu
  • Amélie Dukunde
  • Rolf DanielEmail author
Biotechnologically relevant enzymes and proteins
  • 197 Downloads

Abstract

Owing to the functional versatility and potential applications in industry, interest in lipolytic enzymes tolerant to organic solvents is increasing. In this study, functional screening of a compost soil metagenome resulted in identification of two lipolytic genes, est1 and est2, encoding 270 and 389 amino acids, respectively. The two genes were heterologously expressed and characterized. Est1 and Est2 are thermostable enzymes with optimal enzyme activities at 80 and 70 °C, respectively. A second-order rotatable design, which allows establishing the relationship between multiple variables with the obtained responses, was used to explore the combined effects of temperature and pH on esterase stability. The response curve indicated that Est1, and particularly Est2, retained high stability within a broad range of temperature and pH values. Furthermore, the effects of organic solvents on Est1 and Est2 activities and stabilities were assessed. Notably, Est2 activity was significantly enhanced (two- to tenfold) in the presence of ethanol, methanol, isopropanol, and 1-propanol over a concentration range between 6 and 30% (v/v). For the short-term stability (2 h of incubation), Est2 exhibited high tolerance against 60% (v/v) of ethanol, methanol, isopropanol, DMSO, and acetone, while Est1 activity resisted these solvents only at lower concentrations (below 30%, v/v). Est2 also displayed high stability towards some water-immiscible organic solvents, such as ethyl acetate, diethyl ether, and toluene. With respect to long-term stability, Est2 retained most of its activity after 26 days of incubation in the presence of 30% (v/v) ethanol, methanol, isopropanol, DMSO, or acetone. All of these features indicate that Est1 and Est2 possess application potential.

Keywords

Carboxylesterases Metagenomic library Second-order rotatable design Thermophilic Organic solvent tolerance 

Notes

Acknowledgments

We thank Dr Silja Brady and Sarah Zachmann for providing assistance. We acknowledge the support of Mingji Lu by “Erasmus Mundus Action 2 – Lotus I Project.”

Author contributions

The manuscript was written through the contributions of all authors. All authors have given approval to the final version of the manuscript.

Compliance with ethical standards

This article does not contain any studies with human participants or animals performed by any of the authors.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

253_2019_9695_MOESM1_ESM.pdf (1.8 mb)
ESM 1 (PDF 1797 kb)

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© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Genomic and Applied Microbiology, Göttingen Genomics Laboratory, Institute of Microbiology and GeneticsGeorg-August-University of GöttingenGöttingenGermany

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