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Applied Microbiology and Biotechnology

, Volume 103, Issue 7, pp 3193–3203 | Cite as

Temperature and humidity index (THI)-induced rumen bacterial community changes in goats

  • Shu Zhong
  • Yi Ding
  • Yaoyue Wang
  • Guangchen Zhou
  • Hongran Guo
  • Yulin ChenEmail author
  • Yuxin YangEmail author
Applied microbial and cell physiology

Abstract

The rumen microbiome is thought to play an important role in maintaining normal gastrointestinal metabolism and nutrient absorption in ruminants. The present study was designed to investigate the effect of heat stress on the rumen microbiome of goats using 16S rRNA sequencing technology. Six female goats were randomly allocated into two control metabolic chambers: A and B (in which the temperature and humidity could be precisely controlled with a precision deviation of ± 0.5 °C and ± 5%, with three goats/chamber). Dynamic changes in the rumen bacterial community were detected under 16 gradually increasing temperature and humidity indexes (THIs). Heat stress had no significant effect on alpha diversity but affected the main phyla and genera of the goat rumen microbiota. With a deeper level of heat stress, the TH groups formed a distinct cluster that differed from that of the control check (CK) group. The dominant phylum transitioned from Firmicutes to Bacteroidetes, and co-exclusion occurred between these two phyla. With the increase in THI, the content of probiotics in the Lachnospiraceae_ND3007_group (P < 0.05) decreased, and the abundance of pathogenic bacteria, such as Erysipelotrichaceae_UCG-004 and Treponema_2, increased; however, the difference between the groups was not significant (P > 0.05). Phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) was used to predict bacterial function, and we found that the ambient environment significantly affected the balance between carbohydrate and energy metabolism (P < 0.05). In conclusion, heat stress changed the composition of rumen microbes and affected metabolic function. This experiment provides a theoretical basis for exploring the effects of environmental factors on the rumen of goats.

Keywords

Rumen Microbiome Heat stress Temperature and humidity index (THI) 16S rRNA 

Notes

Acknowledgments

We thank Jinpeng Li and Guojun Wang for their powerful help during sample collection and Yunjia Li for graphics support.

Funding information

This study was financially supported by the National Key Research and Development Program of China (2016YFD0500508) and China Agriculture Research System (CARS-39-12).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All Experimental Animal Management Committee of Northwest A&F University guidelines for the care and experimental procedures of animals were followed.

Supplementary material

253_2019_9673_MOESM1_ESM.pdf (1.5 mb)
ESM 1 (PDF 1.51 mb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Animal Science and TechnologyNorthwest A&F UniversityYanglingChina

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