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Antibacterial effects of iron oxide (Fe3O4) nanoparticles: distinguishing concentration-dependent effects with different bacterial cells growth and membrane-associated mechanisms

  • Lilit Gabrielyan
  • Ashkhen Hovhannisyan
  • Vladimir Gevorgyan
  • Michail Ananyan
  • Armen TrchounianEmail author
Applied microbial and cell physiology
  • 27 Downloads

Abstract

Nowadays, the influence of nanoparticles (NPs) on microorganisms attracts a great deal of attention as an alternative to antibiotics. Iron oxide (Fe3O4) NPs’ effects on Gram-negative Escherichia coli BW 25113 and Gram-positive Enterococcus hirae ATCC 9790 growth and membrane-associated mechanisms have been investigated in this study. Growth specific rate of E. coli was decreased, indicating the bactericidal effect of Fe3O4 NPs. This inhibitory effect of NPs had a concentration-dependent manner. The reactive oxygen species together with superoxide radicals and singlet oxygen formed by Fe3O4 NPs could be the inhibition cause. Fe3O4 NPs showed opposite effects on E. hirae: the growth stimulation or inhibition was observed depending on NPs concentration used. Addition of NPs altered redox potential kinetics and inhibited H2 yield in E. coli; no change in intracellular pH was determined. Fe3O4 NPs decreased H+-fluxes through bacterial membrane more in E. coli than in E. hirae even in the presence of DCCD and increased ATPase activity more in E. hirae than in E. coli. Our results showed that the Fe3O4 NPs demonstrate differentiating effects on Gram-negative and Gram-positive bacteria likely due to the differences in bacterial cell wall structure and metabolic peculiarities. Fe3O4 NPs of different concentrations have no hemolytic (cytotoxic) activity against erythrocytes. Therefore, they can be proposed as antibacterial agents in biomedicine, biotechnology, and pharmaceutics.

Keywords

Antibacterials Nanoparticles Bacterial growth Membrane-associated mechanisms 

Notes

Acknowledgements

We thank Harutyun Sargsyan (Yerevan State University, Yerevan, Armenia) for the help in some experiments, and Shushanik Kazaryan and Lilit Rshtuni (Russian-Armenian University) for preparing NPs. The authors thank Prof. Hrachik Vardapetyan (Russian-Armenian University) for the advances and discussion. Unfortunately, recently he passed away.

Availability of data and materials

All data generated or analyzed during this study are included in this published article.

Authors’ contributions

L.G. performed the main part of experimental work and data processing; A.H. performed experiments with determination of cytotoxicity of NPs and participated in intracellular pH determination; L.G. and A.T. wrote the manuscript; V.G. prepared NPs, was involved in analysis and discussion of data, manuscript revision; M.A. provided TEM and investigated crystalline structure of Fe3O4 NPs; A.T. supervised and coordinated the research, designed the study, edited the manuscript. All authors read and approved the final manuscript.

Funding

This work was supported by Basic support from Russian-Armenian University.

Compliance with ethical standards

Ethics approval and consent to participate

Not applicable.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Medical Biochemistry and BiotechnologyRussian-Armenian UniversityYerevanArmenia
  2. 2.Department of Technology for Materials and Electronic Technique StructuresRussian-Armenian UniversityYerevanArmenia
  3. 3.“Nano-industry” ConcernMoscowRussia

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