Trichosporon fermentans biomass flocculation from soybean oil refinery wastewater using bioflocculant produced from Paecilomyces sp. M2-1
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The soybean oil refinery (SOR) wastewater contains a high concentration of chemical oxygen demand (COD) and lipid, so the direct emissions of SOR wastewater will result in environmental pollution and waste of resources. Oleaginous yeast Trichosporon fermentans can consume organic materials in SOR wastewater to synthesize microbial oil, which achieves the purpose of SOR wastewater resource utilization. The effective harvesting technology of oleaginous yeasts can improve the utilization efficiency. In this study, Paecilomyces sp. M2-1 with high flocculating activity was isolated. The flocculants produced by M2-1 (MBF2-1) include 75% (w/w) polysaccharides, rely on cations, and display the flocculation percentage of above 77% in the range of pH 2–11. Especially under alkaline conditions, the flocculation percentage can be kept above 97%. The results of scanning electron microscope observation and zeta potential measurements suggested that the bridging, net trapping, and sweeping were the main flocculation mechanism of MBF2-1. MBF2-1 could flocculate T. fermentans that was used to reduce the organic matter in SOR wastewater and to produce microbial oil. Under the optimum conditions, the flocculation percentage of MBF2-1 against T. fermentans from SOR wastewater can reach 95%. Fatty acid content percent in microbial oil from T. fermentans was not almost affected by flocculation of MBF2-1. Moreover, MBF2-1 can further remove 55% and 53% of COD and oil content in the fermented SOR wastewater, respectively. The properties and high flocculating percentage displayed by MBF2-1 indicated its potential application prospect in oleaginous yeast harvest and food industry wastewater treatment.
KeywordsBioflocculants Paecilomyces sp. M2-1 Oleaginous yeast harvest Soybean oil refinery wastewater Trichosporon fermentans Flocculation mechanism
This work was funded by National Natural Science Foundation of China (grant number 31470787, 21708003) and Science and Technology Research Project of Jilin Province, China (grant number 20170519015JH).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Dohare D, Meshram R (2014) Biological treatment of edible oil refinery wastewater using activated sludge process and sequencing batch reactors—a review. Int J Eng Sci Res Technol 3(12):251–260Google Scholar
- He J, Zou J, Shao ZZ, Zhang JB, Liu ZD, Yu ZN (2010) Characteristics and flocculating mechanism of a novel bioflocculant HBF-3 produced by deep-sea bacterium mutant Halomonas sp. V3a’. World J Microbiol Biotechnol 26:1135–1141Google Scholar
- Hu YS, Liu B, Hao XD, Cao YL (2015) Current status and outlook of microalgae flocculation in wastewater treatment. Acta Scien Circum 35(1):12–29Google Scholar
- Kandasamy R (2016) Studies on comparison of sludge produced from conventional treatment process and electrochemical processes of soya oil refinery processing wastewater. J Ind Pollut Control 32(2):562–571Google Scholar
- Kurane R, Nohata Y (1991) Microbial flocculation of waste liquids and oil emulsion by a bioflocculant from Alcaligenes latus. J Agric Chem Soc Jpn 55(4):1127–1129Google Scholar
- Liu WJ, Zhao CC, Jiang JH, Lu Q, Hao Y, Wang L, Liu C (2015) Bioflocculant production from untreated corn stover using Cellulosimicrobium cellulans L804 isolate and its application to harvesting microalgae. Biotechnol Biofuels 8:170. https://doi.org/10.1186/s13068-015-0354-4 CrossRefPubMedPubMedCentralGoogle Scholar
- Pu SY, Ma H, Deng DL, Xue SY, Zhu RX, Zhou Y, Xiong XY (2018) Isolation, identification, and characterization of an Aspergillus niger bioflocculant-producing strain using potato starch wastewater as nutrilite and its application. PLoS One 13. https://doi.org/10.1371/journal.pone.0190236
- Qiao N, Gao MX, Fan X (2016) Research progress on the method of separation and recovery of oils and fats used in wastewater treatment. J Northeast Dianli Univ 36(5):63–67Google Scholar
- Takagi H, Kadowaki K (1985) Flocculant production by Paecilomyces sp. taxonomic studies and culture conditions for production. J Agric Chem Soc Jpn 49(11):3151–3157Google Scholar
- Wan L, Tang J, Zhao X (2011) Methods of researching flocculating mechanism of microbial flocculants. Chin J Environ Eng 5(3):481–488Google Scholar
- Wang L, Song ZQ, Li JT, Li XJ, Liu XH, Liang F (2014) Effect of different extraction processes on oil productivity of yeast. China Oils and Fats 39(7):13–16Google Scholar
- Wang W (2009) Synthetic characteristics of bioflocculant by flocculant-producing bacteria and parsing of flocculating active substances. Dissertation, Harbin Institute of TechnologyGoogle Scholar
- Wu D (2017). Analysis of biosynthetic pathway of polysaccharide-type microbial flocculant and population regulatory mechanism. Dissertation, Harbin Institute of TechnologyGoogle Scholar
- Yang TX, Zhang ZP, Zhang X, Tan TW (2014) Comparison of precipitation and flotation methods for the enrichment of Rhodotorula glutinis. J. Beijing. Univ. Chem. Technol. (Nat. Sci. Ed.) 41(1), 78–82Google Scholar
- Yu DY, Wang XN, Fan X, Ren HM, Hu S, Wang L, Shi YF, Liu N, Qiao N (2018) Refined soybean oil wastewater treatment and its utilization for lipid production by the oleaginous yeast Trichosporon fermentans. Biotechnol Biofuels 11:299. https://doi.org/10.1186/s13068-018-1306-6 CrossRefPubMedPubMedCentralGoogle Scholar
- Zhang Q, Ye M (2016) Research on fermentation of exo-polysaccharides from Paecilomyces japonica. Food Res Dev 37(15):145–148Google Scholar
- Zhang Y, Sun XL, Hou YM, Sun Y (2015) Effects of natural oils and surfactants on the production of lipid and lipase with Trichosporon fermentans. J Dalian Polytech Univ 34(3):163–166Google Scholar