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Novel strains of Bacteroides fragilis and Bacteroides ovatus alleviate the LPS-induced inflammation in mice

  • Huizi Tan
  • Jianxin Zhao
  • Hao Zhang
  • Qixiao ZhaiEmail author
  • Wei Chen
Applied microbial and cell physiology

Abstract

Lipopolysaccharide (LPS) can promote the expression of pro-inflammatory cytokines, damage the tight junction of epithelial walls, and thereby lead to chronic low-grade intestinal inflammatory disorders. Evidences of many beneficial functions from Bacteroides strains suggest their intervention capabilities in LPS-induced inflammation. In the present study, both healthy and LPS-treated mice were consistently treated with Bacteroides strains for 5 days. The intestinal microbiota alteration, epithelial permeability, cytokine expression, and autoimmune and innate immune responses were analyzed. B. fragilis HCK-B3 and B. ovatus ELH-B2 from our laboratory collection were demonstrated to assist intestinal equilibrium by maintaining the diversity of gut microbiota and relieve LPS-induced inflammation by either modulating cytokine production or restoring the Treg/Th-17 balance. Our research indicated that the Bacteroides strains with capabilities of alleviating inflammation have the potential as therapeutics to prevent intestinal inflammatory disorders and provided scientific supports for discovering next-generation probiotics.

Keywords

Bacteroides Inflammation Lipopolysaccharide Next-generation probiotics Regulatory T cells 

Notes

Acknowledgements

This work was supported by the Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province. We acknowledge Dr. Pinghu Zhang from China Pharmaceutical University for raising the animals.

Funding

This work was funded by the National Natural Science Foundation of China (No. 31601452), the National Natural Science Foundation of China Key Program (No. 31530056), the Natural Science Foundation of Jiangsu Province (BK20160175), and the National First-Class Discipline Program of Food Science and Technology (JUFSTR20180102).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval

All procedures performed in studies involving animals were approved by the Animal Ethics Committee of China Pharmaceutical University (CPU20170546), and the protocols for the care and use of the mice were based on European Community guidelines (Directive 2010/63/EU).

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Food Science and TechnologyJiangnan UniversityWuxiPeople’s Republic of China
  2. 2.School of Food Science and TechnologyJiangnan UniversityWuxiPeople’s Republic of China
  3. 3.National Engineering Research Center for Functional FoodJiangnan UniversityWuxiPeople’s Republic of China
  4. 4.International Joint Research Laboratory for Probiotics at Jiangnan UniversityWuxiChina
  5. 5.Beijing Innovation Center of Food Nutrition and Human HealthBeijing Technology and Business University (BTBU)BeijingPeople’s Republic of China

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