In vitro and in vivo investigations of probiotic properties of lactic acid bacteria isolated from Chinese traditional sourdough

  • Yang Li
  • Tongjie Liu
  • Minjie Zhao
  • Hao Zhong
  • Wenxin Luo
  • Fengqin FengEmail author
Applied microbial and cell physiology


A total of 88 lactic acid bacteria (LAB) strains were isolated from Chinese traditional sourdough and five of them were selected based on their bile resistance. All the five strains were identified as Lactobacillus plantarum by 16S rRNA gene sequencing. In vitro probiotic properties of the L. plantarum strains including tolerance to simulated gastrointestinal conditions, aggregation activity, and cholesterol removal ability were assessed. Two representatives, L. plantarum ZJUFT34 and L. plantarum ZJUFT17, were intragastrically administered to male C57BL/6J mice of 4-week age for 6 weeks to evaluate their in vivo health-promoting effects. The results indicated that L. plantarum ZJUFT34, L. plantarum ZJUFHN9, and L. plantarum ZJUFAH5 could survive the 3-h incubation in simulated gastric juice with a pH value of 2.0, while L. plantarum ZJUFT32 and L. plantarum ZJUFT17 exhibited better autoaggregation activities and coaggregation activities with pathogens. All the strains showed a cholesterol removal ability in vitro. However, L. plantarum ZJUFT34 or L. plantarum ZJUFT17 administration did not significantly change the serum total cholesterol in vivo. But the ratio of high-density lipoprotein cholesterol to low-density lipoprotein cholesterol was significantly increased by the L. plantarum administration. Besides, L. plantarum ZJUFT17 significantly lowered serum tumor necrosis factor (TNF)-α concentrations. Furthermore, the administration of the LAB strains showed significant influences on lipid metabolism-related gut microbiota. These findings suggested that the L. plantarum strains may benefit the prevention of metabolic syndrome.


Sourdough L. plantarum Serum cholesterol Tumor necrosis factor-α Gut microbiota 



This research was supported by the Hangzhou industry-university cooperation project [grant number 20161631E01] and Agricultural Technology Promotion Special Fund of Zhejiang University New Rural Development Institute [grant number 2017006].

Compliance with ethical standards

All institutional and national guidelines for the care and use of laboratory animals were followed.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

253_2018_9554_MOESM1_ESM.pdf (921 kb)
ESM 1 (PDF 920 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Yang Li
    • 1
    • 2
  • Tongjie Liu
    • 3
  • Minjie Zhao
    • 1
    • 2
  • Hao Zhong
    • 1
    • 2
  • Wenxin Luo
    • 1
  • Fengqin Feng
    • 1
    • 2
  1. 1.College of Biosystems Engineering and Food ScienceZhejiang UniversityHangzhouChina
  2. 2.Zhejiang Key Laboratory for Agro-Food ProcessingZhejiang UniversityHangzhouChina
  3. 3.College of Food Science and EngineeringOcean University of ChinaQingdaoChina

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