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Quorum sensing between Gram-negative bacteria responsible for methane production in a complex waste sewage sludge consortium

  • Phuong Dong Thi Nguyen
  • Nurul Asyifah Mustapha
  • Kiwao Kadokami
  • Rodolfo Garcia-Contreras
  • Thomas K. Wood
  • Toshinari MaedaEmail author
Environmental biotechnology

Abstract

Quorum sensing (QS) plays a key role in activating bacterial functions through small molecules called autoinducers. In this study, the QS of Gram-negative bacteria in waste sewage sludge (WSS) was downregulated by adding the quorum quenching enzyme, AiiM lactonase, which cleaved the acyl-homoserine lactone (AHL) autoinducer signals from Gram-negative bacteria, and subsequently methane production was inhibited by over 400%. The pH was lowered after 2 days in the anaerobic fermentation whereas protease activity at the hydrolysis step was almost the same with or without AiiM. The production of acetic acid significantly increased during the fermentation in the presence of AiiM. The bacterial community at day 2 indicated that the population of Gram-positive bacteria increased in the presence of AiiM, and the percentage of Gram-negative bacteria decreased in the WSS containing AiiM. The change in the bacterial community in the presence of AiiM may be due to the different antimicrobial agents produced in the WSS because some of the Gram-positive bacteria were killed by adding the solid-phase extraction (SPE) fraction from the WSS without AiiM. In contrast, the SPE fraction with AiiM had reduced bactericidal activity against Gram-negative bacteria. Thus, bacterial signaling between Gram-negative bacteria is critical for methane production by the microbial consortia.

Keywords

Anaerobic digestion Waste sewage sludge Quorum sensing AHL lactonase Gram-negative bacteria 

Notes

Acknowledgements

The authors wish to thank Dr. Tomohiro Morohoshi for providing a plasmid harboring an AHL lactonase.

Funding

This study was supported by Kitakyushu City as a research grant.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies performed with human participants or with animals by any of the authors.

Supplementary material

253_2018_9553_MOESM1_ESM.pdf (1.1 mb)
ESM 1 (PDF 1110 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Biological Functions Engineering, Graduate School of Life Science and Systems EngineeringKyushu Institute of TechnologyWakamatsuJapan
  2. 2.Department of Chemical EngineeringThe University of Danang, University of Science and TechnologyDanangVietnam
  3. 3.Department of Chemical and Environmental EngineeringThe University of KitakyushuKitakyushuJapan
  4. 4.Department of Microbiology and Parasitology, Faculty of MedicineUNAMMexico CityMexico
  5. 5.Department of Chemical EngineeringPennsylvania State UniversityUniversity ParkUSA

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