Bacterial tannases: classification and biochemical properties
Tannin acyl hydrolases, also known as tannases, are a group of enzymes critical for the transformation of tannins. The study of these enzymes, which initially evolved in different organisms to detoxify and/or use these plant metabolites, has nowadays become relevant in microbial enzymology research due to their relevant role in food tannin transformation. Microorganisms, particularly bacteria, are major sources of tannase. Cloning and heterologous expression of bacterial tannase genes and structural studies have been performed in the last few years. However, a systematic compilation of the information related to all recombinant tannases, their classification, and characteristics is missing. In this review, we explore the diversity of heterologously produced bacterial tannases, describing their substrate specificity and biochemical characterization. Moreover, a new classification based on sequence similarity analysis is proposed. Finally, putative tannases have been identified in silico for each group of tannases taking advantage of the use of the “tannase” distinctive features previously proposed.
KeywordsTannase Feruloyl esterase Esterase Hydrolyzable tannins Gallic acid
We thank MINEICO for the Severo Ochoa Excellence accreditation (SEV-2016-0644).
This work was financially supported by grants AGL2014-52911-R and SAF2015-73549-JIN from the Spanish Ministry of Economy and Competitiveness/FEDR-MINEICO.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animal performed by any of the authors.
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