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Applied Microbiology and Biotechnology

, Volume 103, Issue 2, pp 893–902 | Cite as

Bile salt hydrolase activity is present in nonintestinal lactic acid bacteria at an intermediate level

  • Xiao Ru
  • Chuang-Chuang Zhang
  • Ya-Hong Yuan
  • Tian-Li Yue
  • Chun-Feng GuoEmail author
Applied microbial and cell physiology
  • 127 Downloads

Abstract

It is generally considered that bile salt hydrolase (BSH) activity is hardly detected in nonintestinal lactic acid bacteria (LAB). The aim of this study was to investigate the distribution and intensity of BSH activity in LAB isolated from naturally fermented vegetables and milk. A total of 624 lactic acid bacterial strains classified into 6 genera and 50 species were isolated from 144 naturally fermented vegetable samples and 103 naturally fermented milk samples, and their BSH activity was screened by gas chromatography with electron capture detection. The BSH-positive strains were further analyzed quantitatively for their deconjugation ability against six human-conjugated bile salts by HPLC based on the disappearance of the conjugated bile salts from the reaction mixture. The results showed that 39% of the strains possessed BSH activity distributed in 24 lactic acid bacterial species. The strains of the fermented vegetable origin showed a 0.5-fold higher incidence of BSH-positive strains than those of the fermented milk origin, and the lactic acid bacilli exhibited 2.5-fold higher incidence of BSH-positive strains than the lactic acid cocci in general. The strains of the fermented vegetable origin generally had greater bile salt deconjugation ability than those of the fermented milk origin. More than 97% and 93% of the BSH-positive strains exhibited a greater substrate preference for glycoconjugated bile salts than tauroconjugated bile salts and for dihydroxy bile salts than trihydroxy bile salts, respectively. This study demonstrated that BSH activity was also present in nonintestinal LAB.

Keywords

Fermented vegetable Fermented milk Lactic acid bacteria Bile salt hydrolase 

Notes

Funding information

This study was funded by the National Natural Science Foundation of China (NSFC, Nos: 31301444, 31501472, 31471638).

Compliance with ethical standards

This article does not contain any studies with human participants or animals performed by any of the authors.

Competing interests

The authors declare that they have no conflict of interests.

Supplementary material

253_2018_9492_MOESM1_ESM.pdf (27 kb)
ESM 1 (PDF 26 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Food Science and EngineeringNorthwest A&F UniversityYanglingChina

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