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Applied Microbiology and Biotechnology

, Volume 103, Issue 1, pp 473–488 | Cite as

Mechanism study of cyfluthrin biodegradation by Photobacterium ganghwense with comparative metabolomics

  • Tengzhou Wang
  • Chaoyang Hu
  • Rongrong Zhang
  • Aili Sun
  • Dexiang Li
  • Xizhi ShiEmail author
Environmental biotechnology
  • 87 Downloads

Abstract

A high-efficiency pyrethroid-degrading bacterium, Photobacterium ganghwense strain 6046 (PGS6046), was first isolated from an offshore seawater environment. Metabolomics method was used to investigate the biotransformation pathway of PGS6046 to cyfluthrin wherein 156 metabolites were identified. The growth rates of the PGS6046 cultivated in nourishing media were much higher than those cultivated in seawater, regardless of the presence of cyfluthrin. Statistical analyses revealed that the metabolic profile of PGS6046 was associated with the culture medium, the presence of cyfluthrin, and culture time. The PGS6046 cultivated in a nourishing medium was characterized by higher levels of amino acids, a lower abundance of intermediates in the tricarboxylic acid cycle, and the presence of some fatty acids than those cultivated in seawater. The effects of cyfluthrin on PGS6046 metabolism varied based on the culture medium, whereas the cyanoalanine levels increased under both culture conditions. Culture time significantly affected the metabolism of amino acids and carbohydrates in PGS6046. The present study revealed the metabolic characteristics of PGS6046 under different culture conditions and will further facilitate the exploration of the fundamental questions regarding PGS6046 and its potential applications in environmental bioremediation.

Keywords

Biodegradation Biotransformation Microbiology Cyfluthrin Metabolomics 

Notes

Acknowledgements

This work was supported by the Zhejiang Provincial Natural Science Foundation of China (LR16C190001), the National Natural Science Foundation of China (No. 31772856), the Technology Innovation Team of Ningbo city (2015C110018), and the K.C. Wong Magna Fund in Ningbo University.

Compliance with ethical standards

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Conflict of interest

Tengzhou Wang declares that he has no conflict of interest. Chaoyang Hu declares that he has no conflict of interest. Rongrong Zhang declares that she has no conflict of interest. Aili Sun declares that she has no conflict of interest. Dexiang Li declares that he has no conflict of interest. Xizhi Shi declares that he has no conflict of interest.

Supplementary material

253_2018_9458_MOESM1_ESM.pdf (877 kb)
ESM 1 (PDF 876 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Tengzhou Wang
    • 1
  • Chaoyang Hu
    • 1
  • Rongrong Zhang
    • 1
  • Aili Sun
    • 1
  • Dexiang Li
    • 1
  • Xizhi Shi
    • 1
    Email author
  1. 1.School of Marine SciencesNingbo UniversityNingboPeople’s Republic of China

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