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Applied Microbiology and Biotechnology

, Volume 103, Issue 1, pp 327–337 | Cite as

Isopropylmalate isomerase MoLeu1 orchestrates leucine biosynthesis, fungal development, and pathogenicity in Magnaporthe oryzae

  • Wei TangEmail author
  • Haolang Jiang
  • Qiaojia Zheng
  • Xuehang Chen
  • Rufeng Wang
  • Shuai Yang
  • Guiyuan Zhao
  • Jiao Liu
  • Justice Norvienyeku
  • Zonghua WangEmail author
Genomics, transcriptomics, proteomics

Abstracts

The biosynthesis of branched-chain amino acids (BCAAs) is conserved in fungi and plants, but not in animals. The Leu1 gene encodes isopropylmalate isomerase that catalyzes the conversion of α-isopropylmalate into β-isopropylmalate in the second step of leucine biosynthesis in yeast. Here, we identified and characterized the functions of MoLeu1, an ortholog of yeast Leu1 in the rice blast fungus Magnaporthe oryzae. The transcriptional level of MoLEU1 was increased during conidiation and in infectious stages. Cellular localization analysis indicated that MoLeu1 localizes to the cytoplasm at all stages of fungal development. Targeted gene deletion of MoLEU1 led to leucine auxotrophy, and phenotypic analysis of the generated ∆Moleu1 strain revealed that MoLeu1-mediated leucine biosynthesis was required for vegetative growth, asexual development, and pathogenesis of M. oryzae. We further observed that invasive hyphae produced by the ∆Moleu1 strain were mainly limited to the primary infected host cells. The application of exogenous leucine fully restored vegetative growth and partially restored conidiation as well as pathogenicity defects in the ∆Moleu1 strain. In summary, our results suggested that MoLeu1-mediated leucine biosynthesis crucially promotes vegetative growth, conidiogenesis, and pathogenicity of M. oryzae. This study helps unveil the regulatory mechanisms that are essential for infection-related morphogenesis and pathogenicity of the rice blast fungus.

Keywords

Magnaporthe oryzae Isopropylmalate isomerase MoLeu1 Asexual development Pathogenicity Leucine biosynthesis 

Notes

Funding information

This research was supported by the Natural Science Foundation of China (31601584), Natural Science Foundation of Fujian Province (2016J05070), Science Fund for Distinguished Young Scholars of Fujian Agriculture and Forestry University to W. T. (KXJQ17020), and China Scholarship Council.

Compliance with ethical standards

This article does not contain any studies with human participants or animals performed by any of the authors.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

253_2018_9456_MOESM1_ESM.pdf (849 kb)
ESM 1 (PDF 848 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Wei Tang
    • 1
    Email author
  • Haolang Jiang
    • 1
  • Qiaojia Zheng
    • 1
  • Xuehang Chen
    • 1
  • Rufeng Wang
    • 1
  • Shuai Yang
    • 2
  • Guiyuan Zhao
    • 1
  • Jiao Liu
    • 1
  • Justice Norvienyeku
    • 1
    • 2
  • Zonghua Wang
    • 1
    • 3
    Email author
  1. 1.State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant ProtectionFujian Agriculture and Forestry UniversityFuzhouChina
  2. 2.State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, College of Life ScienceFujian Agriculture and Forestry UniversityFuzhouChina
  3. 3.Institute of Ocean ScienceMinjiang UniversityFuzhouChina

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