Retrospective evaluation of in vitro effect of gentamicin B1 against Fusarium species
- 150 Downloads
The in vitro susceptibility of gentamicin fractions against Fusarium growth was the subject of this retrospective study. Fusariosis was earlier an exceptionally rare human disease and an unrealistic idea to treat soil saprophytes and plant pathogens with expensive antibiotics such as gentamicins or their minor components. Disseminated fusariosis is now the second most frequent lethal fungal infection after aspergillosis especially in neutropenic patients with hematologic malignancy. Results of this study obtained between May and November 1973 were interesting but not practicable and remained unpublished. Seven Fusarium and 28 other fungal strains were tested for their susceptibility to gentamicin B1. The anti-Fusarium activity of gentamicin B1 was between 0.2 and 3.1 μg/ml minimum inhibitory concentration (MIC) values. The MIC values of clotrimazol and amphotericin B against Fusarium species were significantly higher, 3.1–12.5 μg/ml and 3.1–50 μg/ml, respectively. Gentamicin B1 and its structurally related congeners including hygromycin B, paromomycin, tobramycin (nebramycin factor 5′), nebramycin (nebramycin factor 4), and sisomicin exerted strong in vitro inhibition against Fusarium species between 0.2 and 12.5 μg/ml concentrations. The antibacterial MIC concentration of gentamicin B1 tested on 20 bacterial strains ranged between 0.1 and 50 μg/ml. Gentamicin B1, a minor fraction of the gentamicin complex, inhibited effectively the growth of Gram-positive (Staphylococcus, Streptococcus, Bacillus subtilis) bacteria and Gram-negative (Escherichia coli, Salmonella, Proteus, Pseudomonas) pathogens. Gentamicins and related aminoglycoside antibiotics are used in medical practice. It is proposed that due to the increasing incidence of fusariosis and drug resistance, gentamicin components, particularly minor fraction B1 and related aminoglycoside antibiotics, could be tested for their in vivo activity against fusariosis and aspergillosis either alone or in combination with other antifungal agents.
KeywordsFusariosis Aminoglycoside antibiotics Gentamicins Anti-Fusarium antibiotics Treatment of fusariosis
The author is grateful to Dr. Janos Berdy, Department of Preparative Chemistry, Research Institute of Pharmaceutical Chemistry, who performed the chemical analysis of gentamicin fractions and provided them to the author for antibiotic tests. Gratitude is expressed to Dr. István Koczka, head of the Department of Department of Chemotherapeutics and Antibiotics, for permitting the use of collections of fungal and bacterial strains. The technical assistance of Mrs. Laszlo Ando and Mrs. Otto Szabo is gratefully acknowledged.
Compliance with ethical standards
Conflict of interest
The author declares that he has no conflict of interest.
This article does not contain any studies with human participants performed by the author.
- Ahmad Rather I, Seo BJ, Rejish Kumar VJ, Choi UH, Choi KH, Lim JH, Park YH (2013) Isolation and characterization of a proteinaceous antifungal compound from Lactobacillus plantarum YML007 and its application as a food preservative. Lett Appl Microbiol 57:69–76. https://doi.org/10.1111/lam.12077 CrossRefPubMedGoogle Scholar
- Baradaran-Heravi A, Niesser J, Balgi AD, Choi K, Zimmerman C, South AP, Anderson HJ, Strynadka NC, Bally MB, Roberge M (2017) Gentamicin B1 is a minor gentamicin component with major nonsense mutation suppression activity. Proc Natl Acad Sci U S A 114:3479–3484. https://doi.org/10.1073/pnas.1620982114 CrossRefPubMedPubMedCentralGoogle Scholar
- Dogi CA, Fochesato A, Armando R, Pribull B, de Souza MM, da Silva Coelho I, Araújo de Melo D, Dalcero A, Cavaglieri L (2013) Selection of lactic acid bacteria to promote an efficient silage fermentation capable of inhibiting the activity of Aspergillus parasiticus and Fusarium gramineraum and mycotoxin production. J Appl Microbiol 114:1650–1660. https://doi.org/10.1111/jam.12173 CrossRefPubMedGoogle Scholar
- Gadó I, Bérdy J, Koczka I, Horváth I, Járay M, Zlatos G (1975) Gentamicin antibiotics. Hungarian patent 168,778, Dec. 30, 1973; C.A. 85:19066, 1975Google Scholar
- Haque AK (2018) 12. Fungal diseases in pulmonary pathology (Second Edition), Dani S. Zander and Carol F. Farver (Eds) Elsevier, Oxford, UK, pp. 217–243Google Scholar
- Nucci M, Anaissie EJ, Queiroz-Telles F, Martins CA, Trabasso P, Solza C, Mangini C, Simões BP, Colombo AL, Vaz J, Levy CE, Costa S, Moreira VA, Oliveira JS, Paraguay N, Duboc G, Voltarelli JC, Maiolino A, Pasquini R, Souza CA (2003) Outcome predictors of 84 patients with hematologic malignancies and Fusarium infection. Cancer 98:315–319. https://doi.org/10.1002/cncr.11510 CrossRefGoogle Scholar
- Szigeti ZM, Talas L, Palicz Z, Szentesi P, Hargitai Z, Csernoch L, Balla J, Pocsi I, Banfalvi G, Szeman-Nagy G (2018) Murine model to follow hyphal development in invasive pulmonary aspergillosis. Appl Microbiol Biotechnol 102:2817–2825. https://doi.org/10.1007/s00253-018-8800-4 CrossRefPubMedGoogle Scholar