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Applied Microbiology and Biotechnology

, Volume 102, Issue 21, pp 9159–9170 | Cite as

A novel “trifunctional protease” with reducibility, hydrolysis, and localization used for wool anti-felting treatment

  • Jingxia Mei
  • Nan Zhang
  • Yuanyuan Yu
  • Qiang Wang
  • Jiugang Yuan
  • Ping Wang
  • Li Cui
  • Xuerong Fan
Biotechnologically relevant enzymes and proteins
  • 112 Downloads

Abstract

Proteases can cause unacceptable fiber damage when they are singly applied to wool anti-felting treatment which can make wool textiles machine-washable. Even if protease is attached by synthetic polymers, the modified protease plays a limited role in the degradation of keratin with dense structure consisting of disulfide bonds in the scales. Here, to obtain “machine-washable” wool textiles, a novel “trifunctional protease” with reducibility, hydrolysis, and localization is developed by means of covalent bonding of protease molecules with poly (ethylene glycol) bis (carboxymethyl) ether (HOOC-PEG-COOH) and l-cysteine using carbodiimide/N-hydroxysuccinimide (EDC/NHS) coupling, aiming at selectively degrading the scales on the surface of wool. The formation of polymer is confirmed with size exclusion chromatography (SEC) and Fourier transform infrared spectroscopy (FT-IR). Ellman’s test and fluorescence microscopy reveal that the modified protease can reduce disulfide bonds and restrict hydrolysis of peptide bonds on the wool scales. Furthermore, when applied to wool fabrics, the modified protease reach better treatment effects considering dimensional stability to felting (6.12%), strength loss (11.7%) and scale dislodgement proved by scanning electron microscopy (SEM), alkali solubility, wettability, and dyeability. This multifunctional enzyme is well-designed according to the requirement of the modification of wool surface, showing great potential for eco-friendly functionalization of keratin fibers rich in disulfide linkage.

Keywords

Protease modification PEG wool anti-felting 

Notes

Funding information

This study was funded by the National Key R&D Program of China (2017YFB0309200), the National Natural Science Foundation of China (51673087, 31771039), the Program for Changjiang Scholars and Innovative Research Teams in Universities (IRT_15R26), Fundamental Research Funds for the Central Universities (JUSRP51717A), and national first-class discipline program of Light Industry Technology and Engineering (LITE2018-21).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Supplementary material

253_2018_9276_MOESM1_ESM.pdf (298 kb)
ESM 1 (PDF 297 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Key Laboratory of Science and Technology of Eco-Textile, Ministry of EducationJiangnan UniversityWuxiChina

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